Helicobacter pylori infection and administration of non-steroidal anti-inflammatory drugs down-regulate the expression of gastrokine-1 in gastric mucosa.
Helicobacter pylori infection and administration of non-steroidal anti-inflammatory drugs down-regulate the expression of gastrokine-1 in gastric mucosa.
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DOI：
10.4318/tjg.2012.0345
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发表时间：
2012-06
期刊：
影响因子：
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通讯作者：
W. Mao;Jie Chen;Tie-Li Peng;Xiao-Fei Yin;Lian-Zhou Chen;Minhui Chen
中科院分区：
文献类型：
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作者：
W. Mao;Jie Chen;Tie-Li Peng;Xiao-Fei Yin;Lian-Zhou Chen;Minhui Chen
背景/目的Gastrokine-1是一种在维持胃粘膜完整性中起重要作用的新型蛋白质。然而，是否幽门螺杆菌感染和非甾体抗炎药，这是已知的，导致胃粘膜损伤，影响gastrokine-1在胃粘膜中的表达是未知的。本研究的目的是确定幽门螺杆菌感染或长期服用非甾体类抗炎药患者胃粘膜中gastrokine-1的表达。材料与方法选择40例功能性消化不良患者（20例幽门螺杆菌阴性慢性胃炎，20例幽门螺杆菌阳性慢性胃炎）和37例幽门螺杆菌阴性长期服用非甾体抗炎药者（26例服用阿司匹林，11例服用选择性环氧合酶-2抑制剂）。另外，20名幽门螺杆菌阴性的健康志愿者作为对照。所有受试者均接受了内镜检查，并对胃窦和病变部位进行了活检。通过内镜和组织学观察胃粘膜的变化，并通过免疫组织化学方法分析胃窦粘膜中gastrokine-1蛋白的表达。结果幽门螺杆菌阳性慢性胃炎患者胃动素-1蛋白表达较幽门螺杆菌阴性者和健康对照组明显降低。同样，非甾体抗炎药使用者的gastrokine-1表达也较健康对照组降低，但阿司匹林组和选择性环氧合酶-2抑制剂组gastrokine-1表达无显著差异。此外，gastrokine-1的表达水平往往与慢性胃炎的严重程度相关。结论幽门螺杆菌感染和长期服用非甾体类抗炎药均能下调胃黏膜gastrokine-1的表达，这可能是幽门螺杆菌感染和长期服用非甾体类抗炎药导致胃黏膜损伤的原因之一。
BACKGROUND/AIMS Gastrokine-1 is a novel protein that plays an important role in the maintenance of the integrity of the gastric mucosa. However, whether Helicobacter pylori infection and non-steroidal anti-inflammatory drugs, which are known to cause gastric mucosal injuries, affect gastrokine-1 expression in the gastric mucosa is unknown. The aim of the present study was to determine gastric mucosal expression of gastrokine-1 in patients with Helicobacter pylori infection or long-term non-steroidal anti-inflammatory drug administration. MATERIAL AND METHODS A total of 40 patients with functional dyspepsia (20 with Helicobacter pylori-negative chronic gastritis, and 20 with Helicobacter pylori-positive chronic gastritis), and 37 Helicobacter pylori-negative long-term non-steroidal anti-inflammatory drug users (26 with aspirin, 11 with selective cyclooxygenase-2 inhibitors) were selected. In addition, 20 Helicobacter pylori-negative healthy volunteers were recruited as controls. All subjects underwent endoscopies with biopsies taken from the antrum and the sites with lesions. Gastric mucosal changes were detected endoscopically and histologically, and gastrokine-1 protein expression in the antral mucosa was analyzed by immunohistochemistry. RESULTS Expression of gastrokine-1 protein was decreased in Helicobacter pylori-positive chronic gastritis compared with Helicobacter pylori-negative subjects and the healthy controls. Similarly, gastrokine-1 expression in non-steroidal anti-inflammatory drug users was also decreased, compared with the healthy controls, but there was no significant difference in gastrokine-1 expression between the aspirin group and selective cyclooxygenase-2 inhibitor group. Moreover, gastrokine-1 expression levels tended to be associated with the severity of chronic gastritis. CONCLUSIONS Both Helicobacter pylori infection and long-term non-steroidal anti-inflammatory drug administration downregulate gastrokine-1 expression in the gastric mucosa, which may contribute to the gastric mucosal injuries induced by these two factors.