Noninvasive quantification of regional ventricular function in rats: Assessment of serial change and spatial distribution using ultrasound strain analysis
Noninvasive quantification of regional ventricular function in rats: Assessment of serial change and spatial distribution using ultrasound strain analysis
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DOI：
10.1016/j.echo.2005.01.009
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发表时间：
2005-09-01
影响因子：
6.5
通讯作者：
Beppu, S
中科院分区：
文献类型：
--
作者：
Hirano, T;Asanuma, T;Beppu, S
背景：定量评估大鼠局部心室功能的最佳方法仍不清楚。本研究的目的是探讨超声应变率（SR）和应变分析在评估大鼠局部收缩功能的序列变化和空间分布中的应用。方法：总共 22 只麻醉大鼠接受增量多巴酚丁胺输注（方案 1）以评估系列变化，或接受冠状动脉结扎（方案 2）以评估空间分布。对于方案 1，在后部心肌中测量多巴酚丁胺期间收缩期 SR 和应变的连续变化。在短轴视图上，将收缩应变与壁增厚的百分比变化进行比较。对于方案 2，分析了通过心肌造影超声心动图识别的正常区域、外周缺血区域和中央缺血区域的应变分布的空间分布。结果。在方案 1 中，增量多巴酚丁胺输注导致峰值收缩期 SR 逐渐增加。相比之下，低剂量多巴酚丁胺峰值收缩应变增加，但高剂量多巴酚丁胺则趋于降低。此外，峰值收缩应变的连续变化对应于壁增厚百分比变化的变化，但应变值始终低于壁增厚百分比变化。在方案 2 中，应变曲线表明外周缺血区域出现收缩后增厚，而中央缺血区域出现收缩期壁变薄。结论：超声测定SR和应变是一种准确、无创的定量大鼠局部收缩功能的序列变化和空间分布的方法。
Background: The optimal method for quantitative assessment of regional ventricular function in rats remains unclear. The goal of this study was to investigate the use of ultrasonic strain rate (SR) and strain analysis in evaluating the serial change and spatial distribution of regional contractile function in rats. Methods: In all, 22 anesthetized rats underwent incremental dobutamine infusion (protocol 1) for assessment of serial change or underwent coronary ligation (protocol 2) for assessment of spatial distribution. For protocol 1, the serial change of systolic SR and strain during dobutamine was measured in the posterior myocardium. on the short-axis view, and the systolic strain was compared with the percent change in wall thickening. For protocol 2, the spatial distribution of strain profile was analyzed in normal, peripheral ischemic, and central ischemic regions that were identified by myocardial contrast echocardiography. Results. In protocol 1, the incremental dobutamine infusion resulted in a gradual increase in peak systolic SR. in contrast, peak systolic strain increased with low-dose dobutamine but tended to decrease for higher doses of dobutamine. Further, the serial change of peak systolic strain corresponded to changes in percent change in wall thickening, but the strain values were always lower than percent change in wall thickening. In protocol 2, the strain profile indicated postsystolic thickening in the peripheral ischemic region and indicated systolic wall thinning in the central ischemic region. Conclusions: Ultrasonic determination of SR and strain is an accurate and noninvasive method of quantitation of the serial change and spatial distribution of regional contractile function in rats.