Heterogeneity of Vaginal Microbial Communities within Individuals
Heterogeneity of Vaginal Microbial Communities within Individuals
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DOI：
10.1128/jcm.00854-08
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发表时间：
2009-04-01
影响因子：
9.4
通讯作者：
Wilson, Brenda A.
中科院分区：
文献类型：
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作者：
Kim, Tae Kyung;Thomas, Susan M.;Wilson, Brenda A.
近期不依赖培养的研究表明，健康的阴道生态系统包含着令人惊讶的复杂微生物群落。然而，阴道微生物种群的空间分布和组成尚未使用分子方法进行研究。在此，我们评估了阴道生态系统内特定部位的微生物组成，并研究了采样技术对阴道微生物群检测的影响。从8名临床健康、无症状女性的阴道不同部位（宫颈、穹窿、阴道外口）通过不同方法（擦拭、刮取、灌洗）获取样本，制备了16S rRNA基因克隆文库。数据显示，阴道微生物群在整个阴道内并非均匀分布，就个体而言，在解剖部位和所使用的采样方法方面存在显著差异。因此，这项研究阐明了阴道生态系统的复杂结构，并呼吁在将阴道微生物群作为阴道健康的临床预测指标进行采样时考虑微环境因素。
Recent culture-independent studies have revealed that a healthy vaginal ecosystem harbors a surprisingly complex assemblage of microorganisms. However, the spatial distribution and composition of vaginal microbial populations have not been investigated using molecular methods. Here, we evaluated site-specific microbial composition within the vaginal ecosystem and examined the influence of sampling technique in detection of the vaginal microbiota. 16S rRNA gene clone libraries were prepared from samples obtained from different locations (cervix, fornix, outer vaginal canal) and by different methods (swabbing, scraping, lavaging) from the vaginal tracts of eight clinically healthy, asymptomatic women. The data reveal that the vaginal microbiota is not homogenous throughout the vaginal tract but differs significantly within an individual with regard to anatomical site and sampling method used. Thus, this study illuminates the complex structure of the vaginal ecosystem and calls for the consideration of microenvironments when sampling vaginal microbiota as a clinical predictor of vaginal health.