Effects of high hydrostatic pressure on the dynamic structure of living Escherichia coli membrane: a study using high-pressure time-resolved fluorescence anisotropy measurement
Effects of high hydrostatic pressure on the dynamic structure of living Escherichia coli membrane: a study using high-pressure time-resolved fluorescence anisotropy measurement
复制标题
高静水压对活大肠杆菌膜动态结构的影响：利用高压时间分辨荧光各向异性测量的研究
DOI：
10.1080/08957959.2013.769533
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发表时间：
2013
影响因子：
2
通讯作者：
K.
中科院分区：
文献类型：
--
作者：
Abe;F. and Usui;K.
高静水压力对生物膜的结构有显著的影响。本文利用1-[4-（trimethylamino）phenyl]-6-phenyl-1，3，5-hexatriene（TMA-DPH）时间分辨荧光各向异性测量技术，研究了静水压力对中温大肠杆菌（Escherichia coli）棕榈酰-油酰磷脂酰胆碱（POPC）膜和天然膜的影响。50 MPa的静水压使E.在37°C时，COICELLE膜的扩散系数从0.782降至0.815，旋转扩散系数Dw为0.1MPa时的69.4%。高压对活体也有类似的影响。colicell膜与POPC获得的那些在SandDw方面的变化。因此，只要膜处于液晶相，天然膜响应于高压的动态行为可以部分地从模型膜的研究中推导出。
High hydrostatic pressure has a significant impact on the structure of biological membranes. Here, we elucidated the effects of hydrostatic pressure on a model palmitoyl-oleylphosphatidylcholine (POPC) membrane and a natural membrane of mesophilicEscherichia coliby means of time-resolved fluorescence anisotropy measurement using 1-[4-(trimethylamino)phenyl]-6-phenyl-1,3,5-hexatriene (TMA-DPH). Hydrostatic pressure of 50 MPa increased the order parameterSof theE. colicell membrane from 0.782 to 0.815 at 37°C and it markedly reduced the rotational diffusion coefficientDwof TMA-DPH to 69.4% of that observed at 0.1 MPa. High pressure exerted similar effects on the livingE. colicell membrane to those obtained with POPC in terms of the changes inSandDw. Therefore, the dynamic behavior of the natural membrane in response to high pressure could in part be deduced from studies of the model membrane as long as the membrane is in the liquid-crystalline phase.