Bioprinted Living Coral Microenvironments Mimicking Coral‐Algal Symbiosis
Bioprinted Living Coral Microenvironments Mimicking Coral‐Algal Symbiosis
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生物打印活珊瑚微环境模仿珊瑚与藻类共生
DOI：
10.1002/adfm.202202273
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发表时间：
2022
影响因子：
19
通讯作者：
Xiang, Yi
中科院分区：
文献类型：
--
作者：
Wangpraseurt, Daniel;Sun, Yazhi;You, Shangting;Chua, Sing‐Teng;Noel, Samantha K.;Willard, Helena F.;Berry, David B.;Clifford, Alexander M.;Plummer, Sydney;Xiang, Yi
珊瑚-藻类共生是驱动地球上最壮观的结构之一的生物引擎：珊瑚礁。在这里，活珊瑚微栖息地是使用3D生物打印技术设计的，作为珊瑚藻类共生的仿生模型系统。开发了用于封装珊瑚光共生微藻（Breviolum psygmophilum）的各种生物墨水，并通过3D生物打印珊瑚组织和骨架微尺度特征来模拟珊瑚传质现象。在组织-海水界面，仿生珊瑚虫和结缔组织结构成功地复制了O2扩散边界层的自然建立。在生物打印结构内部，珊瑚状的微型胃腔使用多材料生物打印工艺进行工程设计。在组织下，构建体在微米尺度上模拟珊瑚文石骨架的多孔结构，可以对其进行操作以评估骨骼结构对应力相关过氧化氢（H2 O2）产生的影响。生物打印的活珊瑚微栖息地复制了扩散相关的现象，这些现象是珊瑚藻类共生的功能和破坏的基础，可以用于合成设计师珊瑚的增材制造。
The coral‐algal symbiosis is the biological engine that drives one of the most spectacular structures on Earth: the coral reef. Here, living coral microhabitats are engineered using 3D bioprinting, as biomimetic model system of the coral‐algal symbiosis. Various bioinks for the encapsulation of coral photosymbiotic microalgae (Breviolum psygmophilum) are developed and coral mass transfer phenomena are mimicked by 3D bioprinting coral tissue and skeleton microscale features. At the tissue–seawater interface, the biomimetic coral polyp and connective tissue structures successfully replicate the natural build‐up of the O2diffusive boundary layer. Inside the bioprinted construct, coral‐like microscale gastric cavities are engineered using a multi‐material bioprinting process. Underneath the tissue, the constructs mimic the porous architecture of the coral aragonite skeleton at the micrometer scale, which can be manipulated to assess the effects of skeletal architecture on stress‐related hydrogen peroxide (H2O2) production. The bioprinted living coral microhabitats replicate the diffusion‐related phenomena that underlie the functioning and breakdown of the coral‐algal symbiosis and can be exploited for the additive manufacturing of synthetic designer corals.