Effects of hypoxia on osteogenic differentiation of rat bone marrow mesenchymal stem cells
Effects of hypoxia on osteogenic differentiation of rat bone marrow mesenchymal stem cells
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DOI：
10.1007/s11010-011-1124-7
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发表时间：
2012-03
影响因子：
4.3
通讯作者：
Yating Wang;Juan Li;Yanmin Wang;Lei Lei-Lei;Chunmiao Jiang;S. An;Yuxia Zhan;Qian Cheng;Zhihe Zh
中科院分区：
文献类型：
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作者：
Yating Wang;Juan Li;Yanmin Wang;Lei Lei-Lei;Chunmiao Jiang;S. An;Yuxia Zhan;Qian Cheng;Zhihe Zh
骨重建在正畸治疗中是必不可少的，以骨重建来矫正错牙合。间充质干细胞（MSCs）已被证明具有巨大的成骨能力。本研究旨在探讨缺氧对体外培养大鼠骨髓间充质干细胞成骨过程的影响。在本研究中，我们发现，在成骨诱导后，rBMSCs暂时暴露于缺氧（2%氧气）7天，导致ALPase活性和骨钙素和Runt相关转录因子2/核心结合因子a1 （Runx2/Cbfa1）的表达显著降低。同时，我们发现缺氧暴露导致ERK1/2磷酸化水平早期和短暂性升高，但对丝裂原活化蛋白激酶（p38 MAPK）水平无明显影响。基于这些结果，我们认为缺氧可能通过MEK-ERK 1/2抑制rBMSCs成骨分化，而p38 MAPK可能不参与这一调控。进一步探讨缺氧对骨形成的影响机制，必将为临床实践提供可靠的依据。
Bone reconstruction is essential in orthodontic treatment that caters to the correction of malocclusion by bone reconstruction. Mesenchymal stem cells (MSCs) have been demonstrated a great potency of osteogenesis. The aim of this study was to investigate the effect of hypoxia on the rat bone marrow MSCs (rBMSCs) in vitro during osteogenesis. In this study, we found that temporary exposure of rBMSCs after osteogenic induction for 7 days to hypoxia (2% oxygen) led to a marked decrease in ALPase activity and the expression of osteocalcin and Runt related transcription factor 2/core binding factor a1 (Runx2/Cbfa1). Meanwhile, we found that exposure to hypoxia led to an early and transient increase in the level of phosphorylated ERK1/2 but had no obvious effects on mitogen-activated protein kinase (p38 MAPK) level. Based on these results, we concluded that hypoxia could inhibit osteogenic differentiation of rBMSCs possibly through MEK-ERK 1/2, while p38 MAPK may not participate in this regulation. Further exploration into the mechanisms of hypoxia on osteogenesis would surely provide reliable evidence for clinical practice.