Neuroimmune interactions: role for cholinergic neurons in intestinal anaphylaxis.
Neuroimmune interactions: role for cholinergic neurons in intestinal anaphylaxis.
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神经免疫相互作用：胆碱能神经元在肠道过敏反应中的作用。
DOI：
10.1152/ajpgi.1992.263.6.g847
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Cooke,HJ
中科院分区：
文献类型：
--
作者：
Javed,NH;Wang,YZ;Cooke,HJ
在过敏反应中胆碱能神经元介导氯分泌的作用进行了评估，在肌肉剥离段的远端结肠从豚鼠免疫到牛奶。β-乳球蛋白在免疫组织（而非非免疫组织）中引起短路电流（Isc）的浓度依赖性增加。吡罗昔康、吡拉明和西咪替丁可降低对β-乳球蛋白的Isc反应。河豚毒素和阿托品降低免疫动物对β-乳球蛋白的Isc反应，而美加明和ICS 205-930无效。β-乳球蛋白诱发了免疫动物乙酰胆碱（ACh）释放的浓度依赖性增加，但非免疫动物则不然。在免疫组织中，用β-乳球蛋白攻击后，ACh的释放抑制了Isc的变化。吡罗昔康，西咪替丁加吡拉明，或吡罗昔康，西咪替丁，吡拉明的组合显着减少β-乳球蛋白的挑战后，乙酰胆碱的释放。组胺，二甲双胍，和E2引起ACh释放的增加。这些结果表明，β-乳球蛋白可能从肥大细胞释放β-lactoglobulin和组胺。当免疫动物用β-乳球蛋白攻击时发生的分泌应答部分地由胆碱能神经元的激活引起，所述胆碱能神经元利用毒蕈碱突触将信号传递到上皮。
The role of cholinergic neurons in mediating chloride secretion in anaphylaxis was assessed in muscle-stripped segments of distal colon from guinea pigs immunized to bovine milk. beta-Lactoglobulin evoked a concentration-dependent increase in short-circuit current (Isc) in immune, but not nonimmune, tissues. The Isc response to beta-lactoglobulin was reduced by piroxicam, pyrilamine, and cimetidine. Tetrodotoxin and atropine reduced the Isc response to beta-lactoglobulin in immune animals, whereas mecamylamine and ICS 205-930 were ineffective. beta-Lactoglobulin evoked a concentration-dependent increase in acetylcholine (ACh) release in immune, but not nonimmune, animals. In immune tissues after challenge with beta-lactoglobulin, ACh release paralleled the change in Isc. Piroxicam, cimetidine plus pyrilamine, or a combination of piroxicam, cimetidine, and pyrilamine significantly reduced the release of ACh after beta-lactoglobulin challenge. Histamine, dimaprit, and prostaglandins E2 evoked an increase in ACh release. These results suggest that beta-lactoglobulin releases prostaglandins and histamine probably from mast cells. Secretory responses that occur when immune animals are challenged with beta-lactoglobulin result, in part, from activation of cholinergic neurons that utilize muscarinic synapses for transfer of signals to the epithelium.