Efficient incorporation of galactose into lipopolysaccharide by Escherichia coli K-12 strains with polar galE mutations.
Efficient incorporation of galactose into lipopolysaccharide by Escherichia coli K-12 strains with polar galE mutations.
复制标题
具有极性 galE 突变的大肠杆菌 K-12 菌株将半乳糖有效掺入脂多糖。
DOI：
10.1128/jb.172.9.5511-5513.1990
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发表时间：
1990
影响因子：
3.2
通讯作者：
Austin,EA
中科院分区：
文献类型：
--
作者：
Schnaitman,CA;Austin,EA
在插入galE::Tn10的菌株和缺失galOPE的菌株中观察到外源半乳糖与脂多糖的有效结合。在galETK缺失较长的菌株中没有观察到合并，这表明合并到galE突变体中是由于内部启动子或偶然启动子的galTK表达水平较低。这些galE突变体为脂多糖的放射性标记提供了一种方便、特异的方法。
Efficient incorporation of exogenous galactose into lipopolysaccharide was observed in a strain with a galE::Tn10 insertion and a strain with a deletion of galOPE. No incorporation was observed in a strain with a longer (galETK) deletion, indicating that incorporation into the galE mutants was due to a low level of expression of galTK from internal or fortuitous promoters. These galE mutants provided a convenient and specific way to radiolabel lipopolysaccharide.