Patch clamp study of mouse glomus cells using a whole carotid body.
Patch clamp study of mouse glomus cells using a whole carotid body.
复制标题
使用整个颈动脉体对小鼠血管球细胞进行膜片钳研究。
DOI：
10.1016/j.neulet.2003.10.062
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Shirahata,Machiko
中科院分区：
文献类型：
--
作者：
Yamaguchi,Shigeki;Lande,Boris;Kitajima,Toshimitsu;Hori,Yuichi;Shirahata,Machiko
Some electrophysiological characteristics of mouse glomus cells (DBA/2J strain) were investigated using an undissociated carotid body. The carotid body with major carotid arteries was placed in a recording chamber, and glomus cells were visualized with a water immersion lens combined with an infrared differential interference video camera. Patch clamp experiments revealed that voltage-gated outward current, but not inward current, was easily observed in glomus cells. Pharmacological experiments and the kinetics of the current suggest that outward current is via delayed rectifier, A type, and large conductance calcium-activated K channels. Furthermore, K current was reversibly attenuated by mild hypoxia. The results suggest electrophysiological similarities of glomus cells among the cat, the rat, and the DBA/2J mouse. The method appears useful for physiological experiments.
