Active screen plasma nitriding enhances cell attachment to polymer surfaces
Active screen plasma nitriding enhances cell attachment to polymer surfaces
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DOI：
10.1016/j.apsusc.2013.03.001
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发表时间：
2013-05-15
影响因子：
6.7
通讯作者：
Stamboulis, Artemis
中科院分区：
文献类型：
--
作者：
Kaklamani, Georgia;Bowen, James;Stamboulis, Artemis
活性屏等离子体渗氮（ASPN）是一种用于材料表面改性的成熟技术，其结果通常是产品具有增强的功能性能。在这里，我们报告了超高分子量聚乙烯（UHMWPE）的化学和机械性能的修改，使用80：20（v/v）N-2/H-2 ASPN，然后在处理和未处理的聚合物表面上生长3 T3成纤维细胞。ASPN处理的UHMWPE在接种后3小时内显示出广泛的成纤维细胞附着，而成纤维细胞未成功附着到未处理的UHMWPE上。成纤维细胞包被的表面保持长达28天，监测其代谢活性和形态。使用X射线光电子能谱研究了ASPN处理的UHMWPE表面的化学性质，揭示了C-N、C=N和C N化学键的存在。在环境条件下样品储存期间以及在两种常用细胞培养基中浸泡期间，研究了ASPN处理的UHMWPE表面的弹性模量、表面形貌和粘附特性超过28天。(c)2013爱思唯尔有限公司版权所有。
Active screen plasma nitriding (ASPN) is a well-established technique used for the surface modification of materials, the result of which is often a product with enhanced functional performance. Here we report the modification of the chemical and mechanical properties of ultra-high molecular weight poly(ethylene) (UHMWPE) using 80:20 (v/v) N-2/H-2 ASPN, followed by growth of 3T3 fibroblasts on the treated and untreated polymer surfaces. ASPN-treated UHMWPE showed extensive fibroblast attachment within 3 h of seeding, whereas fibroblasts did not successfully attach to untreated UHMWPE. Fibroblast-coated surfaces were maintained for up to 28 days, monitoring their metabolic activity and morphology throughout. The chemical properties of the ASPN-treated UHMWPE surface were studied using X-ray photoelectron spectroscopy, revealing the presence of C-N, C=N, and C N chemical bonds. The elastic modulus, surface topography, and adhesion properties of the ASPN-treated UHMWPE surface were studied over 28 days during sample storage under ambient conditions and during immersion in two commonly used cell culture media. (c) 2013 Elsevier B.V. All rights reserved.