Expression of MicroRNAs in Patients With Pancreatic Cancer and Its Prognostic Significance
Expression of MicroRNAs in Patients With Pancreatic Cancer and Its Prognostic Significance
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DOI：
10.1097/mpa.0b013e3182592ba7
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发表时间：
2013-01-01
期刊：
影响因子：
2.9
通讯作者：
Voros, Dionysios
中科院分区：
文献类型：
--
作者：
Papaconstantinou, Ioannis G.;Manta, Asimina;Voros, Dionysios
目的：胰腺癌中已建立的microRNA表达谱的研究及其与临床病理因素的相关性方法：通过实时聚合酶链反应分析88例导管胰腺癌和98例对照样本的miR-21、miR-31、miR-122、miR-145、miR-146 a、miR-155、miR-210和miR-222表达。结果：miR-21、miR-155、miR-210、miR-221和miR-222在正常组织中的表达高于正常组织，而miR-31、miR-122、miR-145和miR-146 a在正常组织中的表达低于正常组织。结论：miR-21和miR-155在乳腺癌中的作用已得到证实，而miR-31、miR-145和miR-146 a的表达下调与目前的文献存在争议，因此有必要对microRNA作为肿瘤生物学标志物的使用进行修订。
Objectives: Investigation of expression profile of well-established microRNAs in pancreatic adenocarcinoma, and its correlation with clinicopathological factors.Methods: Eighty-eight samples of ductal pancreatic adenocarcinoma and 98 control samples were analyzed by real-time polymerase chain reaction for miR-21, miR-31, miR-122, miR-145, miR-146a, miR-155, miR-210, and miR-222 expressions. The results were normalized and then statistically analyzed using nonparametric statistical tests.Results: According to our results, miR-21, miR-155, miR-210, miR-221, and miR-222, were overexpressed in diseased tissues than in the control samples, whereas miR-31, miR-122, miR-145, and miR-146a were underexpressed. Additionally, the expressions of miR-21 and miR-155 were associated with tumor stage and poor prognosis.Conclusions: The tumorigenic role of miR-21 and miR-155 was confirmed, whereas down-regulation of miR-31, miR-145, and miR-146a, in dispute with current literature, renders necessary the revision of use of microRNAs as biological markers.