Hesperidin, a citrus flavonoid, inhibits bone loss and decreases serum and hepatic lipids in ovariectomized mice
Hesperidin, a citrus flavonoid, inhibits bone loss and decreases serum and hepatic lipids in ovariectomized mice
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DOI：
10.1093/jn/133.6.1892
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发表时间：
2003-06-01
影响因子：
4.2
通讯作者：
Ishimi, Y
中科院分区：
文献类型：
--
作者：
Chiba, H;Uehara, M;Ishimi, Y
本研究的目的是研究橙皮苷是否能抑制卵巢切除小鼠（OVX）的骨质流失，OVX是一种绝经后骨质疏松症的动物模型。将48只8周龄雌性ddY小鼠分为5组：假手术组饲喂对照饲料（AIN-93G）， OVX组饲喂对照饲料（OVX组），OVX+ HesA组饲喂含有0.5 g/100 g橙皮苷的对照饲料，OVX+ HesB组饲喂含有0.7 g/100 g α -葡萄糖橙皮苷的对照饲料，OVX+17 β -雌二醇（E-2）组饲喂对照饲料，并用微渗透泵给予0.03杯E-2/d。4周后处死小鼠，立即取血液、股骨、子宫和肝脏标本。橙皮苷对子宫重量无影响。OVX小鼠股骨骨密度低于假手术组（P < 0.05），饲料中橙皮苷或α -葡萄糖橙皮苷可显著预防这种骨质流失。橙皮苷组和E-2组大鼠股骨中Ca、P、Zn浓度显著高于OVX组。组织形态学分析显示，OVX可显著降低股骨远端干骺端骨小梁体积和骨小梁厚度（P < 0.05）， α -葡萄糖橙皮苷可显著防止骨小梁体积和骨小梁厚度的减少。此外，橙皮苷减少了OVX小鼠股骨干骺端破骨细胞的数量，E-2也是如此。食用含橙皮苷饲料的小鼠血清和肝脏脂质低于对照组（P < 0.05）。这些结果表明，柑橘类黄酮在预防与生活方式有关的疾病中可能发挥作用，因为它们对骨骼和脂质有益。中华医学杂志。33(3):1892- 187,2003。
The purpose of this study was to examine whether hesperidin inhibits bone loss in ovariectomized mice (OVX), an animal model of postmenopausal osteoporosis. Forty 8-wk-old female ddY mice were assigned to five groups: a sham-operated group fed the control diet (AIN-93G), an OVX group fed the control diet, an OVX+ HesA group fed the control diet containing 0.5 g/100 g hesperidin, and an OVX+ HesB group fed the control diet containing 0.7 g/100 g alpha-glucosylhesperidin and an OVX+17beta-estradiol (E-2) group fed the control diet and administered 0.03 mug E-2/d with a mini-osmotic pump. After 4 wk, the mice were killed and blood, femoral, uterine and liver were sampled immediately. Hesperidin administration did not affect the uterine weight. In OVX mice, the bone mineral density of the femur was lower than in the sham group (P < 0.05) and this bone loss was significantly prevented by dietary hesperidin or alpha-glucosylhesperidin. The Ca, P and Zn concentrations in the femur were significantly higher in the hesperidin-fed and E-2 groups than in the OVX group. Histomorphometric analyses showed that the trabecular bone volume and trabecular thickness in the femoral distal metaphysis were markedly decreased (P < 0.05) by OVX, and alpha-glucosylhesperidin significantly prevented this bone loss. Furthermore, hesperidin decreased the osteoclast number of the femoral metaphysis in OVX mice, as did E-2. Serum and hepatic lipids were lower in mice that consumed the hesperidin-containing diets (P < 0.05) than in the OVX group fed the control diet. These results suggest a possible role for citrus flavonoids in the prevention of lifestyle-related diseases because of their beneficial effects on bone and lipids. J. Nutr. 133: 1892-1897, 2003.