Specific Extraction of Sialic-acid-containing Glycans and Glycopeptides Using Serotonin-bonded Silica
Specific Extraction of Sialic-acid-containing Glycans and Glycopeptides Using Serotonin-bonded Silica
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DOI：
10.2116/analsci.26.75
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发表时间：
2010-01
影响因子：
1.6
通讯作者：
Masahiro Yodoshi;Tomoko Ikuta;Yukie Mouri;Shigeo Suzuki
中科院分区：
文献类型：
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作者：
Masahiro Yodoshi;Tomoko Ikuta;Yukie Mouri;Shigeo Suzuki
开发了血清素键合硅胶，用于含硫酸的聚糖和糖肽的特异性提取。5-羟色胺通过其乙氨基与醛二氧化硅颗粒还原胺化偶联，对聚糖链末端的唾液酸具有强亲和力。捕获在血清素二氧化硅颗粒上的唾液酸化聚糖通过用醋酸铵溶液洗涤而释放，提供高效的特异性捕获和释放糖缀合物。吸附容量>100 μmol/g，颗粒适用于用2-氨基吡啶衍生化后纯化标记聚糖以去除过量试剂。5-羟色胺硅胶可有效富集胰蛋白酶解物中含唾液酸的糖肽，用于糖蛋白中聚糖异质性的LC/MS分析。
Serotonin-bonded silica was developed for specific extraction of sialic-acid-containing glycans and glycopeptides. Serotonin, coupled by reductive amination with aldehyde silica particles via its ethylamino group, has strong affinity to sialic acid in glycan chain termini. Sialylated glycans trapped on serotonin silica particles are released by washing with ammonium acetate solution, providing highly efficient specific trap and release glycoconjugates. With >100 μmol/g adsorption capacity, the particles are applicable to purify labeled glycans after derivatization with 2-aminopyridine to remove excess reagents. Serotonin silica efficiently enriches sialic-acid-containing glycopeptides from tryptic digests for LC/MS analysis of glycans’ heterogeneity in glycoproteins.