Enhanced radical scavenging activity of genetically modified Arabidopsis seeds
Enhanced radical scavenging activity of genetically modified Arabidopsis seeds
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DOI：
10.1007/s10529-005-0683-7
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发表时间：
2005-03-01
影响因子：
2.7
通讯作者：
Saito, K
中科院分区：
文献类型：
--
作者：
Tohge, T;Matsui, K;Saito, K
拟南芥（Arabidopsis thaliana）pap 1-D突变体种子中原花青素（PA）含量增加，该突变体中编码Myb样转录因子的内源性PAP 1基因被激活标签的花椰菜花叶病毒35 S启动子增强子序列诱导表达。与此相反，PA含量下降，在转基因植物的种子转化与PAP 1 cDNA或与PAP 1嵌合阻遏物，虽然可溶性花青素的量增加过表达PAP 1 cDNA的转基因植物的种子。增强的自由基清除活性只观察到在pap 1-D突变体的种子提取物，表明PAs是主要负责种子中的自由基清除活性。本研究表明，工程的黄酮类化合物生物合成的转录因子的健康有益的植物种子的可行性。
The proanthocyanidin ( PA) content was increased in seeds of pap1-D mutant of Arabidopsis thaliana, in which the expression of endogenous PAP1 gene encoding a Myb-like transcription factor was induced by activation-tagging with enhancer sequences derived from cauliflower mosaic virus 35S promoter. In contrast, the PA contents decreased in seeds of transgenic plants transformed with a PAP1 cDNA or with a PAP1 chimeric repressor, although the amount of soluble anthocyanins increased in seeds of transgenic plants over-expressing PAP1 cDNA. The enhanced radical scavenging activity was observed only in the seed extracts of pap1-D mutant, indicating that PAs are primarily responsible for radical scavenging activity in seeds. The present study suggests the feasibility of engineering a transcription factor of flavonoid biosynthesis for health beneficial plant seeds.