High-cell-density phorbol ester and retinoic acid upregulate involucrin and downregulate suprabasal keratin 10 in autocrine cultures of human epidermal keratinocytes.
High-cell-density phorbol ester and retinoic acid upregulate involucrin and downregulate suprabasal keratin 10 in autocrine cultures of human epidermal keratinocytes.
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DOI：
10.1006/mcbr.1999.0165
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发表时间：
1999-08-01
期刊：
影响因子：
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通讯作者：
Pittelkow, M R
中科院分区：
文献类型：
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作者：
Poumay, Y;Herphelin, F;Pittelkow, M R
人表皮角质形成细胞的自分泌生长是在缺乏外源性生长因子的亚融合细胞培养中开始的，培养基中钙浓度低，细胞密度足够。培养融合抑制角质细胞增殖，上调早期角质蛋白10 （K10）和晚期角质蛋白的表达，这是分化的标志。在本报告中，在高密度（融合后）下研究了自分泌角质形成细胞的表型，特别是在用12- o -十四烷醇- 13-醋酸酯（TPA）或全反式维甲酸（RA）处理后。汇流后，K10降低，而involucrin不降低。TPA上调亚融合角化细胞中天合蛋白的表达，但不上调K10的表达。融合角化细胞与RA治疗下调K10，但上调天合蛋白。与其他体外培养模型不同，该模型首次模拟了RA的体内效应，RA是类视黄醇家族的一员，可有效调节角质形成细胞的分化和选定基因产物的表达。因此，它可以进一步研究表皮分化。
Autocrine growth of human epidermal keratinocytes is initiated in subconfluent cell cultures in the absence of exogenous growth factors, at low calcium concentration of the medium and with sufficient cell density. Culture confluence inhibits keratinocyte proliferation and upregulates expression of early, keratin 10 (K10), and late, involucrin, markers of differentiation. In this report, the phenotype of autocrine keratinocytes was studied at high cell density (postconfluence), specifically after treatment with 12-O-tetradecanoylphorbol 13-acetate (TPA), or all-trans retinoic acid (RA). At postconfluence, K10 is decreased but not involucrin. TPA upregulates involucrin expression, but not K10 in subconfluent keratinocytes. Treatment of confluent keratinocytes with RA downregulates K10, but upregulates involucrin. This in vitro culture model, unlike others, simulates for the first time the in vivo effects of RA, a member of the retinoid family which potently modulates keratinocyte differentiation and the expression of selected gene products. It thus can be developed to further examine epidermal differentiation.