INDUCTION OF ANTIGEN-SPECIFIC CYTOLYTIC T-CELLS IN-SITU IN HUMAN-MELANOMA BY IMMUNIZATION WITH SYNTHETIC PEPTIDE-PULSED AUTOLOGOUS ANTIGEN-PRESENTING CELLS
INDUCTION OF ANTIGEN-SPECIFIC CYTOLYTIC T-CELLS IN-SITU IN HUMAN-MELANOMA BY IMMUNIZATION WITH SYNTHETIC PEPTIDE-PULSED AUTOLOGOUS ANTIGEN-PRESENTING CELLS
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DOI：
10.1073/pnas.92.17.8078
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发表时间：
1995-08-15
影响因子：
11.1
通讯作者：
MAURI, F
中科院分区：
文献类型：
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作者：
MUKHERJI, B;CHAKRABORTY, NG;MAURI, F
人类黑色素瘤细胞可以处理MAGE-1基因产物，并将处理后的九肽EADPTGHSY呈现在其主要组织相容性复合体I类分子HLA-A1上，作为细胞溶解T淋巴细胞(CTL)的决定簇，考虑到合成的非肽冲击的自体抗原提呈细胞(APC)可能是免疫原性的，因此，肿瘤细胞表达MAGE-1基因且HLA-A1(+)的黑色素瘤患者用人工培养的自体APC冲击合成的非肽制成的疫苗免疫。对疫苗体内宿主免疫反应性质的分析表明，多肽冲击的APC能够在免疫部位和远处转移疾病部位原位诱导自体黑色素瘤反应和非肽特异性CTL。
Human melanoma cells can process the MAGE-1 gene product and present the processed nonapeptide EADPTGHSY on their major histocompatibility complex class I molecules, HLA-A1, as a determinant for cytolytic T lymphocytes (CTLs), Considering that autologous antigen presenting cells (APCs) pulsed with the synthetic nonapeptide might, therefore, be immunogenic, melanoma patients whose tumor cells express the MAGE-1 gene and who are HLA-A1(+) were immunized with a vaccine made of cultured autologous APCs pulsed with the synthetic nonapeptide. Analyses of the nature of the in vivo host immune response to the vaccine revealed that the peptide-pulsed APCs are capable of inducing autologous melanoma-reactive and the nonapeptide-specific CTLs in situ at the immunization site and at distant metastatic disease sites.