Spectroscopic studies on the interaction of pradimicin BMY-28864 with mannose derivatives
Spectroscopic studies on the interaction of pradimicin BMY-28864 with mannose derivatives
复制标题
DOI：
10.1093/glycob/8.4.407
复制
发表时间：
1998-04-01
期刊：
影响因子：
4.3
通讯作者：
Lee, YC
中科院分区：
文献类型：
--
作者：
Fujikawa, K;Tsukamoto, Y;Lee, YC
Pradimicin BMY-28864（Pm）是一种对酵母菌和真菌有效的抗生素，已知在Ca ~（2+）存在下与甘露糖结合。我们用光谱法研究了Pm、Ca ~（2+）、甘露糖和甘露糖寡糖之间的相互作用模式（Man alpha 1-OMe，Man alpha 1-2Man alpha 1-OMe，Man alpha 1-3Man alpha 1-OMe，Man alpha 1-4Man alpha 1-OMe，Man alpha 1-6Man alpha 1-OMe，Man alpha 1-6（Man alpha 1-3）Man alpha 1-OMe，和Man（9）GlcNAc（2）-Asn，一种高甘露糖型的N-连接寡糖）。吸光度变化相对于碳水化合物浓度非线性发生，并且不遵循简单的结合等温线方程，这表明了独特的多步相互作用模式。对于单甘露糖苷和二甘露糖苷，诱导最大吸光度变化的一半的浓度类似于10 mM，对于三甘露糖苷和Man（9）GlcNAc（2）-Asn，甲基α-D-吡喃葡萄糖苷，甲基α-D-吡喃半乳糖苷、乳糖和肌醇在50 mM以下不影响Pm的吸光度，单独的Ca 2+也影响Pm的吸光度，当加入Ca 2+时，200和700 nm之间的吸光度呈减色性降低，的浓度，让一半的最大吸光度减少所造成的Ca 2+是约15 μ M我们的结果表明，两个Pm分子结合一个Ca 2+，和每个Pm结合两个甘露糖残基。
Pradimicin BMY-28864 (Pm) is an antibiotic effective against yeasts and fungi, and is known to bind mannose in the presence of Ca2+, We examined spectroscopically the mode of interactions among Pm, Ca2+, and glycosides of mannose and mannose oligosaccharides (Man alpha 1-OMe, Man alpha 1-2Man alpha 1-OMe, Man alpha 1-3Man alpha 1-OMe, Man alpha 1-4Man alpha 1-OMe, Man alpha 1-6Man alpha 1-OMe, Man alpha 1-6(Man alpha 1-3)Man alpha 1-OMe, and Man(9)GlcNAc(2)-Asn, a high mannose type N-linked oligosaccharide), All the mannosides interacted with Pm in the presence of Ca2+ and caused absorbance changes. The absorbance changes occurred nonlinearly with respect to the carbohydrate concentration and do not follow a simple binding isotherm equation, suggesting a unique multistep interaction mode, The concentrations that induced half the maximum absorbance change were similar to 10 mM for the mono-and di-mannosides and around 1.5 mM for the trimannoside and Man(9)GlcNAc(2)-Asn, Methyl alpha-D-glucopyranoside, methyl alpha-D-galactopyranoside, lactose, and myo-inositol did not affect the absorbance of Pm up to 50 mM, Ca2+ alone also influenced the absorbance of Pm, The absorbance between 200 and 700 nm decreased hypochromically when Ca2+ was added, The concentration that gave half the maximum absorbance decrease caused by Ca2+ was around 15 mu M Our results suggest that two Pm molecules bind one Ca2+, and each Pm binds two mannosyl residues.