Efficacy of genital T cell responses to herpes simplex virus type 2 resulting from immunization of the nasal mucosa
Efficacy of genital T cell responses to herpes simplex virus type 2 resulting from immunization of the nasal mucosa
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DOI：
10.1016/j.virol.2003.10.010
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发表时间：
2004-01-20
期刊：
影响因子：
3.7
通讯作者：
Young, CG
中科院分区：
文献类型：
--
作者：
Milligan, GN;Dudley-McClain, KL;Young, CG
阴道内(静脉注射)或鼻内(静脉注射)用胸苷激酶缺陷型单纯疱疹病毒2型(HSV-2)株(tk-)免疫C57BL/6J(136)小鼠，对生殖器上皮和感觉神经节具有相似的保护作用。相比之下，与静脉注射免疫的B细胞缺陷的木乃伊小鼠相比，免疫的木乃伊小鼠对这些位点的保护作用大大减弱。在HSV-2攻击后，免疫的MUMA小鼠生殖器上皮细胞中HSV特异性T细胞比静脉注射免疫的MUMA小鼠要少。被动地将HSV特异性血清转移到免疫MUMA小鼠，恢复了这些部位对HSV-2攻击的保护。这些结果表明，I.N.可能对生殖器和神经元部位起保护作用。但可能更多地依赖于抗体依赖机制，而不是生殖器免疫产生的保护。这些结果对免疫策略有意义，以获得对生殖道和感觉神经节的高水平细胞介导保护。(C)2003 Elsevier Inc.保留所有权利。
Intravaginal (ivag) or intranasal (i.n.) immunization of C57BL/6J (136) mice with a thymidine kinase-deficient strain (tk-) of herpes simplex virus type 2 (HSV-2) resulted in comparable protection of the genital epithelium and sensory ganglia against HSV-2 challenge. In contrast, protection of these sites was much reduced in i.n.-immunized compared to ivag-immunized B cell-deficient muMT mice. Fewer HSV-specific T cells were detected in the genital epithelium of i.n.-immunized compared to ivag-immunized muMT mice after HSV-2 challenge. Passive transfer of HSV-specific serum to immune muMT mice restored protection of these sites against HSV-2 challenge. These results suggest that protection of genital and neuronal sites may be conferred by i.n. immunization but may be more dependent on antibody-dependent mechanisms than the protection resulting from genital immunization. These results have implications for immunization strategies to elicit high levels of cell-mediated protection of the genital tract and sensory ganglia. (C) 2003 Elsevier Inc. All rights reserved.