Bamboo saccharification through cellulose solvent-based biomass pretreatment followed by enzymatic hydrolysis at ultra-low cellulase loadings
Bamboo saccharification through cellulose solvent-based biomass pretreatment followed by enzymatic hydrolysis at ultra-low cellulase loadings
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DOI：
10.1016/j.biortech.2009.09.081
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发表时间：
2010-07-01
影响因子：
11.4
通讯作者：
Zhang, Yi-Heng Percival
中科院分区：
文献类型：
--
作者：
Sathitsuksanoh, Noppadon;Zhu, Zhiguang;Zhang, Yi-Heng Percival
将改性纤维素溶剂（浓磷酸）和有机溶剂（95%乙醇）的木质纤维素分级（COSLIF）应用于自然干燥的毛竹样品。生物质的溶解条件为50 ℃，1大气压60分钟。葡聚糖消化率为88.2%，在超低纤维素酶负载的一个滤纸单位每克葡聚糖。总的葡萄糖和木糖产率分别为86.0%和82.6%。COSLIF有效地破坏了竹子的原纤维结构，导致纤维素酶（CAC）的纤维素可及性从0.27增加到9.14 m2/g生物质，增加了33倍。成本分析表明，当纤维素酶成本高于每加仑纤维素乙醇0.15美元时，使用昂贵的纤维素酶的15倍减少对于降低生物质糖化的总成本将是重要的。(C)2009爱思唯尔有限公司保留所有权利。
The modified cellulose solvent- (concentrated phosphoric acid) and organic solvent- (95% ethanol) based lignocellulose fractionation (COSLIF) was applied to a naturally-dry moso bamboo sample. The biomass dissolution conditions were 50 degrees C, 1 atm for 60 min. Glucan digestibility was 88.2% at an ultra-low cellulase loading of one filter paper unit per gram of glucan. The overall glucose and xylose yields were 86.0% and 82.6%, respectively. COSLIF efficiently destructed bamboo's fibril structure, resulting in a 33-fold increase in cellulose accessibility to cellulase (CAC) from 0.27 to 9.14 m(2) per gram of biomass. Cost analysis indicated that a 15-fold decrease in use of costly cellulase would be of importance to decrease overall costs of biomass saccharification when cellulase costs are higher than $0.15 per gallon of cellulosic ethanol. (C) 2009 Elsevier Ltd. All rights reserved.