Morphological Analysis of Schlemm's Canal in Mice.
Morphological Analysis of Schlemm's Canal in Mice.
复制标题
小鼠Schlemm的运河的形态分析。
DOI：
10.1007/978-1-4939-8712-2_10
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Quaggin SE
中科院分区：
文献类型：
--
作者：
Thomson BR;Quaggin SE
免疫荧光共聚焦显微镜是分析血管和淋巴组织形态和发育的有力工具。施累姆氏管（SC）是眼睛前房中的大的、类似血管的血管，其对于维持眼内压所需的房水引流是必不可少的，并且对血液和淋巴管信号传导途径中的缺陷敏感。在这里，我们描述了一种方法来染色和量化SC面积和形态在去核小鼠的眼睛，提供了一个工具，了解其发展和功能的小动物遗传或疾病模型。
Immunofluorescent confocal microscopy is a powerful tool for analysis of the morphology and development of vascular and lymphatic tissues. Schlemm’s canal (SC) is a large, lymphatic-like vessel in the anterior chamber of the eye, which is essential for aqueous humor drainage required to maintain intraocular pressure and is sensitive to defects in blood and lymphatic vascular signaling pathways. Here, we describe a method to stain and quantify SC area and morphology in enucleated mouse eyes, providing a tool for understanding its development and function in small animal genetic or disease models.