Difference by Frequencies on Cell Killing Effect by Superposing Low Frequency on high frequency Bipolar Burst Pulse
Difference by Frequencies on Cell Killing Effect by Superposing Low Frequency on high frequency Bipolar Burst Pulse
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高频双极突发脉冲叠加低频对细胞杀伤效果的频率差异
DOI：
10.1109/tps.2020.3033968
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发表时间：
2020
影响因子：
1.5
通讯作者：
Sunao Katsuki
中科院分区：
文献类型：
--
作者：
Hiromi Sato;Yasushi Minamitani;Gen Urabe;Taichi Kamezaki;Shuji Okamoto;Sunao Katsuki
超短脉冲高电场提供了新的生物应用，例如包装食品的灭菌。在这项研究中，我们研究了高频和高强度的突发脉冲对酵母细胞的作用，目的是杀死包装食品中的细胞。以前，我们表明，对细胞施加突发脉冲比重复提供单个脉冲更有效地导致细胞死亡。然而，在单极猝发脉冲的情况下，除了要研究的高频分量之外，还包括由猝发脉冲宽度引起的低频分量。因此，尚不清楚哪种频率对杀死细胞有效。在此，通过比较具有低频成分的单极短脉冲、不具有低频成分的双极短脉冲、以及作为单极短脉冲的低频成分的单一脉冲，来研究叠加在高频短脉冲上的低频成分对细胞死亡的影响。结果，通过向细胞施加包括作为高频成分的150-MHz频带和作为低频成分的15-MHz频带的150-MHz频带单极突发脉冲，细胞死亡率高于通过向细胞施加仅具有150-MHz频带的频率成分的双极突发脉冲。此外，通过向细胞施加150-MHz频带单极短脉冲，细胞死亡率高于通过向细胞施加作为150-MHz频带单极短脉冲的低频分量的15-MHz频带单脉冲。这些结果表明，通过在高频上叠加低频，猝发脉冲可以更有效地引起细胞死亡。
An ultrashort pulse high electric field provides new biological applications, such as sterilization of a packaged food. In this study, we investigate the effect on yeast cells by a high-frequency and high-intensity burst pulse for the purpose of killing cells in packaged food. Previously, we showed that applying a burst pulse to cells was more effective in causing cell death than repeatedly supplying a single pulse. However, in case of a unipolar burst pulse, a low-frequency component caused by a burst pulsewidth was included in addition to the high-frequency component to be investigated. Therefore, it was not clear which frequency was effective in killing cells. Here, we investigate how the low-frequency component superposed on the high-frequency burst pulse affects cell death by comparing the unipolar burst pulse having the-low frequency component, the bipolar burst pulse not having the low-frequency component, and a single pulse that was the low-frequency component of the unipolar burst pulse. As a result, the cell death ratio was higher by applying 150-MHz band unipolar burst pulses including 150-MHz band as a high-frequency component and 15-MHz band as a low-frequency component to the cells than by applying bipolar burst pulses with only frequency component of 150-MHz band to the cells. Also, the cell death ratio was higher by applying the 150-MHz band unipolar burst pulses to the cells than by applying the 15-MHz band single pulse that was the low-frequency component of the 150-MHz band unipolar burst pulse to the cells. These results show that the burst pulse is possible in causing cell death more effectively by superposing low frequency on high frequency.