Co-culture with lung cancer A549 cells promotes the proliferation and migration of mesenchymal stem cells derived from bone marrow.
Co-culture with lung cancer A549 cells promotes the proliferation and migration of mesenchymal stem cells derived from bone marrow.
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DOI：
10.3892/etm.2017.4909
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发表时间：
2017-10
影响因子：
2.7
通讯作者：
Xie XD
中科院分区：
文献类型：
--
作者：
Zhang YM;Zhang ZM;Guan QL;Liu YQ;Wu ZW;Li JT;Su Y;Yan CL;Luo YL;Qin J;Wang Q;Xie XD
The initiation and progression of various types of tumors, such as lung neoplasms, are driven by a population of cells with stem cell properties and their microenvironment. Bone marrow mesenchymal stem cells (BM-MSCs) in long-term in vitro culture may exhibit spontaneous changes in stem cell biological properties, including malignant transformations; however, the molecular mechanisms of this have not been fully elucidated. In the present study, a BM-MSC and lung cancer A549 cell co-culture system was utilized to investigate how the tumor microenvironment may spontaneously change the proliferation, migration and differentiation of BM-MSCs. It was demonstrated that the lung cancer A549 microenvironment is able to induce changes in the cell morphology, proliferation, karyotype, cytoskeleton and migration ability of BM-MSCs in vitro. Compared with the control group BM-MSCs, the expression of Ras, phosphorylated-extracellular regulated protein kinases, nuclear factor-κB, P62 and B-cell lymphoma 2 (Bcl-2) proteins in groups of co-cultured BM-MSCs increased significantly (P<0.05) and the expression of P53, Bcl-2 associated X protein and caspase-3 protein decreased significantly (P<0.05). The mechanisms responsible for the changes observed in BM-MSCs may be related to abnormal expression of related genes in the ERK signaling pathway.
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影响因子：
11.2
作者：
Nakamizo, A;Marini, F;Lang, FF
通讯作者：
Lang, FF
影响因子：
4.8
作者：
Maitra, B;Szekely, E;Koç, O
通讯作者：
Koç, O
影响因子：
6.2
作者：
El-Serafi, Mostafa M.;Bahnassy, Abeer A.;Zekri, Abdel-Rahman N.
通讯作者：
Zekri, Abdel-Rahman N.
影响因子：
5.7
作者：
Bergfeld SA;Blavier L;DeClerck YA
通讯作者：
DeClerck YA
影响因子：
64.5
作者：
Das, Sanjeev;Raj, Lakshmi;Lee, Sam W.
通讯作者：
Lee, Sam W.