Whole-Genome Comparative and Pathogenicity Analysis ofSalmonella entericasubsp.entericaSerovar Rissen
Whole-Genome Comparative and Pathogenicity Analysis ofSalmonella entericasubsp.entericaSerovar Rissen
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肠沙门氏菌肠亚种Rissen血清型的全基因组比较及致病性分析
DOI：
10.1534/g3.120.401201
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Wu Wenjuan
中科院分区：
文献类型：
--
作者：
Zhou Aiping;Li Jun;Xu Zhihong;Ni Jinjing;Guo Jian;Yao Yu-Feng;Wu Wenjuan
沙门氏菌是一种已知会引起食源性疾病的细菌。它们的宿主范围差异很大，它们对宿主的敏感性决定了其致病性。肠道沙门氏菌 Rissen 血清型（S. Rissen）是一种分布广泛的血清型；然而，人们对它的毒力和致病性知之甚少。在本研究中，对代表性 S. Rissen 分离株的致病性和抗生素耐药性进行了研究。细胞模型结果显示，与鼠巨噬细胞RAW264.7相比，S. Rissen更喜欢在人巨噬细胞U937中复制，表明其具有一定程度的宿主适应性。基因组测序和比对分析表明，里森沙门氏菌毒力因子的分布和非同义单核苷酸多态性与伤寒沙门氏菌相似，而与鼠伤寒沙门氏菌毒力因子的分布和非同义单核苷酸多态性相似。综上所述，我们的结果表明，尽管里森沙门氏菌是分布在猪群、猪肉和鸡肉产品中的常见血清型，但它具有很强的感染人类的能力。
Salmonella are a type of bacteria known to cause food-borne illness. Their host range varies widely, and their susceptibility to the host determines its pathogenicity. Salmonella enterica serovar Rissen (S. Rissen) is a widely distributed serotype; however, its virulence and pathogenicity are poorly understood. In this study, the pathogenicity and antibiotic resistance of a representative S. Rissen isolate were investigated. The cell model results showed that S. Rissen preferred to replicate in human macrophage cells U937 compared to murine macrophage cells RAW264.7, suggesting that it has a level of host adaptability. Genome sequencing and comparison analysis revealed that the distribution and nonsynonymous single nucleotide polymorphisms of virulence factors in S. Rissen were similar to those in S. Typhi rather than to those in S. Typhimurium. Taken together, our results suggest that although S. Rissen is a common serotype distributed in swine herds, pork and chicken products, it has strong ability to infect humans.