Evidence for multiple interspecific hybridization in Saccharomyces sensu stricto species.
Evidence for multiple interspecific hybridization in Saccharomyces sensu stricto species.
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严格酵母菌种中多种种间杂交的证据。
DOI：
10.1111/j.1567-1364.2002.tb00051.x
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发表时间：
2002
影响因子：
3.2
通讯作者：
Ganter,PhilipF
中科院分区：
文献类型：
--
作者：
deBarrosLopes,Miguel;Bellon,JenniferR;Shirley,NeilJ;Ganter,PhilipF
荧光扩增片段长度多态性分析表明，高水平的基因交换betteramyomyomyosensu stricto物种，与一些菌株经历了多个种间杂交事件，随后的变化，基因组的复杂性。两株啤酒酵母菌系为酵母菌与异源倍体酵母菌的杂交种。模式菌株CBS 380 T的基因组结构与S.酵母基因转移结果表明，苹果酒酵母，CID1，拥有核DNA从三个不同的物种。交配实验表明，单倍体细胞的种间接合没有障碍。此外，异源多倍体菌株能够进行进一步的杂交与otherapeumomyelicensu stricto酵母。这些结果表明，thecomyomyosensu stricto物种之间的渐渗是可能的。
Fluorescent amplified fragment length polymorphism analysis demonstrates a high level of gene exchange betweenSaccharomycessensu stricto species, with some strains having undergone multiple interspecific hybridization events with subsequent changes in genome complexity. Two lager strains were shown to be hybrids betweenSaccharomyces cerevisiaeand the alloploid speciesSaccharomyces pastorianus. The genome structure of CBS 380T, the type strain ofSaccharomyces bayanus, is also consistent withS. pastorianusgene transfer. The results indicate that the cider yeast, CID1, possesses nuclear DNA from three separate species. Mating experiments show that there are no barriers to interspecific conjugation of haploid cells. Furthermore, the allopolyploid strains were able to undergo further hybridizations with otherSaccharomycessensu stricto yeasts. These results demonstrate that introgression between theSaccharomycessensu stricto species is likely.