In TH2 cells the Il4 gene has a series of accessibility states associated with distinctive probabilities of IL-4 production
In TH2 cells the Il4 gene has a series of accessibility states associated with distinctive probabilities of IL-4 production
复制标题
DOI：
10.1073/pnas.162360199
复制
发表时间：
2002-08-06
影响因子：
11.1
通讯作者：
Paul, WE
中科院分区：
文献类型：
--
作者：
Guo, LY;Hu-Li, J;Paul, WE
TH2克隆可以产生非常不同数量的IL-4。在用Gfp取代ll4第一个外显子的纯合或杂合小鼠制备的6个TH2克隆中，观察到ll4/ll3位点CpG甲基化的一系列模式与IL-4或绿色荧光蛋白的表达程度相关。组蛋白乙酰化模式也显示出“高”和“低”TH2克隆之间的差异。这些结果表明，在TH2细胞中，ll4位点可能显示与不同程度的IL-4表达相关的可变染色质可及性模式。这一发现表明，IL-4的表达调控与该细胞因子在细胞/细胞相互作用和阈值反应调控中的功能有关。
TH2 clones may produce very variable amounts of IL-4. Among six TH2 clones prepared from homozygous or heterozygous mice in which Gfp replaced the first exon of ll4, a range of patterns of CpG methylation in the ll4/ll3 locus was observed correlating with the degree of expression of IL-4 or green fluorescence protein. Patterns of histone acetylation also showed differences between "high" and "low" TH2 clones. These results indicate that in TH2 cells the ll4 locus may display variable patterns of chromatin accessibility associated with distinct degrees of IL-4 expression. This finding suggests a regulation of IL-4 expression keyed to the function of this cytokine in cell/cell interactions and in the regulation of threshold responses.