Development and validation of a method for precise dating of female puberty in laboratory rodents: The puberty ovarian maturation score (Pub-Score).
Development and validation of a method for precise dating of female puberty in laboratory rodents: The puberty ovarian maturation score (Pub-Score).
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DOI：
10.1038/srep46381
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发表时间：
2017-04-12
影响因子：
4.6
通讯作者：
Tena-Sempere M
中科院分区：
文献类型：
--
作者：
Gaytan F;Morales C;Leon S;Heras V;Barroso A;Avendaño MS;Vazquez MJ;Castellano JM;Roa J;Tena-Sempere M
青春期是一个关键的发育事件，其主要调控机制仍然知之甚少。青春期的精确测年对于其复杂的神经内分泌控制网络的实验(临床前)研究至关重要。在雌性实验啮齿动物中，青春期的外部标志，如阴道开口(VO)和上皮细胞角化(即第一次阴道发情，FE)，间接地与卵巢的成熟状态和第一次排卵有关，这是青春期的明确标志。然而，在大鼠中，VO和FE几乎与第一次排卵同时发生，而在小鼠中，这些事件没有那么密切的关联。此外，在某些实验条件下，青春期的外部迹象可以与两个物种的首次排卵分开。在此，我们建议将青春期卵巢成熟评分(Pub-Score)作为一种新的、可靠的方法来评估大鼠和小鼠的青春期卵巢成熟。这种方法建立在对青春期前卵巢成熟的组织学评估的基础上，基于有腔卵泡的发育和第一次排卵的准确时间，通过追溯黄体成熟和退行性变化的年代。这种方法允许两个物种在VO后至少两周的时间窗口内准确地确定青春期的时间，从而便于在各种实验条件下识别和精确测定青春期提前或延迟。
Puberty is a key developmental event whose primary regulatory mechanisms remain poorly understood. Precise dating of puberty is crucial for experimental (preclinical) studies on its complex neuroendocrine controlling networks. In female laboratory rodents, external signs of puberty, such as vaginal opening (VO) and epithelial cell cornification (i.e., first vaginal estrus, FE), are indirectly related to the maturational state of the ovary and first ovulation, which is the unequivocal marker of puberty. Whereas in rats, VO and FE are almost simultaneous with the first ovulation, these events are not so closely associated in mice. Moreover, external signs of puberty can be uncoupled with first ovulation in both species under certain experimental conditions. We propose herein the Pubertal Ovarian Maturation Score (Pub-score), as novel, reliable method to assess peripubertal ovarian maturation in rats and mice. This method is founded on histological evaluation of pre-pubertal ovarian maturation, based on antral follicle development, and the precise timing of first ovulation, by retrospective dating of maturational and regressive changes in corpora lutea. This approach allows exact timing of puberty within a time-window of at least two weeks after VO in both species, thus facilitating the identification and precise dating of advanced or delayed puberty under various experimental conditions.