In Vivo Evaluation of Calcium-Phosphate Ceramics with Highly-Interconnected Pores Using Porcine Tibia Defect Model
In Vivo Evaluation of Calcium-Phosphate Ceramics with Highly-Interconnected Pores Using Porcine Tibia Defect Model
复制标题
使用猪胫骨缺陷模型对具有高度互连孔的磷酸钙陶瓷进行体内评估
DOI：
10.4028/p-coiscj
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
M. Aizawa
中科院分区：
文献类型：
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作者：
Yusuke Shigemitsu;H. Nagashima;H. Matsunari;M. Aizawa
本研究的目的是比较具有相似多孔结构的羟基磷灰石(HAP)和β-磷酸三钙(β-TcP)陶瓷在成骨方面的差异。将磷酸钙纤维与碳珠混合(~150μm)烧成了具有高度互连结构的多孔HAP和HAP-TCP陶瓷(总孔隙率为~70%)。用压汞技术测量高度连通的孔洞大小。将每个样本(ϕ4 mm×8 mm)植入猪(2岁，雄性)的胫骨缺损处(ϕ4.2 mm×8 mm)。植入6周后制备不脱钙切片，苏木精-伊红(HE)染色。评估了新骨面积和剩余材料面积，羟基磷灰石和β-tCP陶瓷的互连尺寸分别为23.0µm和15.6µm。β-TCP陶瓷的新骨面积明显高于羟基磷灰石陶瓷。β-TCP陶瓷的残料率为77.1±3.3%，而羟基磷灰石陶瓷的残料率保持稳定。在HE染色切片上，与β-TCP陶瓷相比，羟基磷灰石陶瓷的成骨不均匀。虽然β-TcP陶瓷的连接尺寸较小，但较高的吸收率可能对同质骨的形成起到了积极的作用。根据研究结果，β-TCP制备的高度互连的多孔陶瓷可能是一种适合骨再生的材料。
The aim of this study was to compare the difference in bone formation between hydroxyapatite (HAp) and β-tricalcium phosphate (β-TCP) ceramics with similar porous structure. Porous HAp and β-TCP ceramics (total porosity: ~70%) with highly-interconnected structure were fabricated by firing calcium-phosphate fiber mixed with carbon beads (~150 μm). The size of highly-interconnected pores was measured by mercury intrusion technique. Each sample (ϕ4 mm × 8 mm) was implanted into the tibial bone defect (ϕ4.2 mm × 8 mm) of a pig (2 years old, male). After 6 weeks of implantation, undecalcified section was prepared and stained with hematoxylin and eosin (HE) stain. New bone area and remaining material area were evaluated.Interconnection sizes of HAp and β-TCP ceramics were 23.0 µm and 15.6 µm, respectively. New bone area of β-TCP ceramics was significantly higher compared to HAp ceramics. Remaining material ratio was 77.1 ± 3.3% for β-TCP ceramics whereas HAp ceramics remained stable. Bone formation in HAp ceramics was inhomogeneous compared to β-TCP ceramics in HE stained section. Although the β-TCP ceramics interconnection size was smaller, higher resorption may have played a positive role in homogeneous bone formation. According to the result of the study, it is suggested that highly-interconnected porous ceramics made by β-TCP could be a suitable material for bone regeneration.
可生物降解的β-磷酸三钙/聚(L-乳酸)杂化物的制备及其体外评价
DOI：
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发表时间：
2007
期刊：
Archives of BioCeramics Research 7
影响因子：
--
作者：
Y. Iwamoto;T. Miyashita;Y. Tanaka;N. Sugiyama;Y. Takeoka;M. Rikukawa;M. Aizawa
通讯作者：
M. Aizawa