EXPRESSION OF MANY DEVELOPMENTALLY REGULATED GENES IN MYXOCOCCUS DEPENDS ON A SEQUENCE OF CELL-INTERACTIONS
EXPRESSION OF MANY DEVELOPMENTALLY REGULATED GENES IN MYXOCOCCUS DEPENDS ON A SEQUENCE OF CELL-INTERACTIONS
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DOI：
10.1101/gad.1.8.840
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发表时间：
1987-10-01
影响因子：
10.5
通讯作者：
KAISER, D
中科院分区：
文献类型：
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作者：
KROOS, L;KAISER, D
黄色粘球菌的某些发育突变体可以通过野生型细胞在细胞外补充。这些突变体的表现就好像它们在发育所需的细胞-细胞相互作用中有缺陷。可能存在几种不同的相互作用，因为突变体属于四种胞外互补组（A、B、C和D）。我们在这里报告，B-和C-突变改变粘球菌发育过程中的基因表达模式检测到转录融合到lacZ介导的Tn 5乳糖。突变C基因座减少或消除了发育β-半乳糖苷酶的表达，从15乳糖融合，通常开始表达野生型细胞后6小时的发展。通过添加野生型细胞，使这些C-依赖性lac融合体的表达恢复至C-突变体。C-突变不影响通常在发育6小时前开始表达的10个lac融合体的表达，表明C-介导的细胞-细胞相互作用需要在发育约6小时开始。细胞在发育的非常早期需要B+功能，因为B-突变减少或消除了来自所有26个测试的lac融合体的发育β-半乳糖苷酶表达，包括一些通常开始在发育开始时表达的融合体。在C-突变体和B-突变体中，一些乳糖融合体的β-半乳糖苷酶表达降低，而在发育过程中通常开始β-半乳糖苷酶表达的其他融合体不表达β-半乳糖苷酶，这表明在给定的时间类别内发育基因对细胞-细胞相互作用的缺乏表现出不同的敏感性。对B+和C+功能的要求，以及先前描述的A+功能，似乎位于相同的发育途径上。
Certain developmental mutants of Myxococcus xanthus can be complemented extracellularly by wild-type cells. These mutants behave as if they are defective in cell-cell interactions that are required for development. There may be several different interactions because the mutants belong to four extracellular complementation groups (A, B, C, and D). We report here that B- and C- mutations change the pattern of gene expression during Myxococcus development as detected by transcriptional fusions to lacZ mediated by Tn5 lac. The mutant C locus reduced or abolished developmental beta-galactosidase expression from 15 lac fusions that normally begin to be expressed in wild-type cells after 6 hr of development. Expression of these C-dependent lac fusions was restored to C- mutants by adding wild-type cells. The C- mutation did not affect the expression of 10 lac fusions that normally begin to be expressed before 6 hr of development, indicating that the C-mediated cell-cell interaction is required beginning at about 6 hr of development. Cells require the B+ function very early in development because a B- mutation reduced or abolished developmental beta-galactosidase expression from all 26 lac fusions tested, including some that normally begin to be expressed at the onset of development. In a C- mutant and in a B- mutant, some lac fusions responded with reduced beta-galactosidase expression, whereas other fusions, which would normally begin beta-galactosidase expression at about the same time during development, expressed no beta-galactosidase, indicating that developmental genes within a given temporal class display different sensitivities to the absence of cell-cell interactions. Requirements for B+ and C+ function, as well as the previously described A+ function, appear to lie on the same developmental pathway.