Effect of TU-100 on Peyer's patches in a bacterial translocation rat model.
Effect of TU-100 on Peyer's patches in a bacterial translocation rat model.
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TU-100对细菌移位大鼠模型中派尔集合淋巴结的影响。
DOI：
10.1002/ags3.12460
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发表时间：
2021-09
影响因子：
2.7
通讯作者：
Shimada M
中科院分区：
文献类型：
--
作者：
Takasu C;Miyazaki K;Yoshikawa K;Nishi M;Tokunaga T;Kashihara H;Yoshimoto T;Ogawa H;Morine Y;Shimada M
Daikenchuto（TU-100）是一种日本草药，广泛用于治疗各种胃肠道疾病。我们以前曾报道过TU-100通过维持微生物组的多样性来抑制CPT-11诱导的细菌易位（BT）。在这项研究中，我们表明TU-100通过诱导派伊尔集合淋巴结中的PD-1表达来调节BT期间的免疫应答。将18只雄性Wistar大鼠分为4组：对照组;对照+ TU-100组，经口给予TU-100 1000 mg/kg，持续5天; BT组，腹膜内给予CPT-11 250 mg/kg，持续2天; TU-100组，经口给予TU-100 1000 mg/kg，持续5天，并在第4天和第5天腹膜内给予CPT-11 250 mg/kg。与对照组相比，BT组的Peyer集合淋巴结大小显著更大（9.0 × 104 µm2 vs 29.4 × 104 µm2，P < .05），但TU-100组有所改善（15.4 × 104 µm2，P < .005）。与对照组和BT组相比，TU-100显著诱导派尔集合淋巴结中的PD-1表达（对照vs BT vs TU-100 = 4.3 ± 4.9 vs 5.1 ± 10.3 vs 17.9 ± 17.8）。与对照组相比，BT组的CD 4+细胞增加（P <0.05），但TU-100组减少。与对照组相比，BT组中的Foxp 3+细胞增加（P <0.05），并且与BT组相比，TU-100组中的Foxp 3+细胞进一步增加。CPT-11显著增加BT组TLR 4、NF-κβ、TNF-α mRNA表达。TU-100共处理显著逆转了这些mRNA表达。TU-100可能通过派伊尔集合淋巴结中的PD-1表达对BT具有保护作用。Daikenchuto（TU‐100）是一种日本草药，广泛用于各种胃肠道疾病。我们以前曾报道过TU-100通过维持微生物组的多样性来抑制CPT-11诱导的细菌易位（BT）。在这项研究中，我们表明TU-100通过诱导派伊尔集合淋巴结中的PD-1表达来调节BT期间的免疫应答。
Daikenchuto (TU‐100), a Japanese herbal medicine, is widely used for various gastrointestinal diseases. We have previously reported that TU‐100 suppresses CPT‐11‐induced bacterial translocation (BT) by maintaining the diversity of the microbiome. In this study we show that TU‐100 modulates the immune response during BT by inducing PD‐1 expression in Peyer's patches. Eighteen male Wistar rats were divided into four groups: a control group; a control + TU‐100 group, given TU‐100 1000 mg/kg orally for 5 d; a BT group, given CPT‐11 250 mg/kg intra‐peritoneal for 2 d; and a TU‐100 group, given TU‐100 1000 mg/kg orally for 5 d with CPT‐11 250 mg/kg intra‐peritoneal on days 4 and 5. The size of Peyer's patch was significantly bigger in the BT group compared to the control group (9.0 × 104 µm2 vs 29.4 × 104 µm2, P < .05), but improved in the TU‐100 group (15.4 × 104 µm2, P < .005). TU‐100 significantly induced PD‐1 expression in Peyer's patch compared to the control group and the BT group (control vs BT vs TU‐100 = 4.3 ± 4.9 vs 5.1 ± 10.3 vs 17.9 ± 17.8). The CD4+ cells were increased in the BT group (P < .05) compared to the control group but decreased in the TU‐100 group. The Foxp3+ cells were increased in the BT group compared to the control group (P < .05), and further increased in the TU‐100 group compared to the BT group. CPT‐11 significantly increased TLR4, NF‐κβ, TNF‐α mRNA expressions in the BT group. TU‐100 cotreatment significantly reversed these mRNA expressions. TU‐100 may have a protective effect against BT through PD‐1 expression in Peyer's patch. Daikenchuto (TU‐100), a Japanese herbal medicine, is widely used for various gastrointestinal diseases. We have previously reported that TU‐100 suppresses CPT‐11‐induced bacterial translocation (BT) by maintaining the diversity of the microbiome. In this study, we show that TU‐100 modulates the immune response during BT by inducing PD‐1 expression in Peyer's patches.
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