Macromolecular structure of cellulose studied by second-harmonic generation imaging microscopy
Macromolecular structure of cellulose studied by second-harmonic generation imaging microscopy
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DOI：
10.1364/ol.28.002207
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发表时间：
2003-11-15
期刊：
影响因子：
3.6
通讯作者：
Campagnola, PJ
中科院分区：
文献类型：
--
作者：
Brown, RM;Millard, AC;Campagnola, PJ
纯化的纤维素样品的大分子结构的二次谐波产生（SHG）成像显微镜进行了研究。我们发现，在Valonia和醋酸杆菌纤维素的SHG对比强烈类似于从动物组织的胶原蛋白，无论是在形态和偏振各向异性。偏振分析表明，每个片层中的微纤维高度排列和有序，并且在相邻片层中改变方向，90度。二次谐波强度的角度依赖性很好地符合cos（2）θ分布，这是电偶极相互作用的特征。Valonia纤维素酶降解纤维素酶其次是在真实的时间SHG成像和指数衰减动力学的结果，表明SHG成像显微镜是理想的监测动态生物系统。2003年美国光学学会。
The macromolecular structure of purified cellulose samples is studied by second-harmonic generation (SHG) imaging microscopy. We show that the SHG contrast in both Valonia and Acetobacter cellulose strongly resembles that of collagen from animal tissues, both in terms of morphology and polarization anisotropy. Polarization analysis shows that microfibrils in each lamella are highly aligned and ordered and change directions by, 90degrees in adjacent lamellae., The angular dependence of the SHG intensity fits well to a cos(2)theta distribution, which is characteristic of the electric dipole interaction. Enzymatic degradation of Valonia fibers by cellulase is followed in real time by SHG imaging and results in exponential decay kinetics, showing that SHG imaging microscopy is ideal for monitoring dynamics in biological systems. 2003 Optical Society of America.