Systematic comparison of CRISPR/Cas9 and RNAi screens for essential genes.
Systematic comparison of CRISPR/Cas9 and RNAi screens for essential genes.
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DOI：
10.1038/nbt.3567
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发表时间：
2016-06
影响因子：
46.9
通讯作者：
Bassik MC
中科院分区：
文献类型：
--
作者：
Morgens DW;Deans RM;Li A;Bassik MC
我们比较了shRNA和CRISPR/Cas9筛选在人类慢性粒细胞白血病细胞系K562中识别必需基因的能力。我们发现两个文库在检测必需基因方面的精度是相似的，并且结合来自两个筛选的数据可以提高性能。值得注意的是，两种筛选的结果几乎没有相关性，这可以通过每种技术识别不同的基本生物过程来部分解释。
We compare the ability of shRNA and CRISPR/Cas9 screens to identify essential genes in the human chronic myelogenous leukemia cell line K562. We find that the precision of the two libraries in detecting essential genes is similar and that combining data from both screens improves performance. Notably, results from the two screens show little correlation, which can be partially explained by identification of distinct essential biological processes with each technology.