Microcapillary Chip-Based Extracellular Vesicle Profiling System
Microcapillary Chip-Based Extracellular Vesicle Profiling System
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DOI：
10.1007/978-1-4939-7253-1_17
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发表时间：
2017-01-01
期刊：
影响因子：
--
通讯作者：
Ichiki, Takanori
中科院分区：
文献类型：
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作者：
Akagi, Takanori;Ichiki, Takanori
描述了一种基于微毛细管芯片的用于表征细胞外囊泡（EV）的粒子电泳系统。到目前为止，在技术上很难分析或识别范围从几十到一百纳米的颗粒的异质群体，因此，在研究细胞外囊泡的研究人员中，对纳米颗粒的新分析方法的需求不断增长。本章介绍的分析平台允许检测直径小于50 nm的单个纳米颗粒或纳米囊泡，并能够基于多个指标（如浓度、直径、zeta电位和表面抗原性）表征纳米颗粒。该平台将为获得用于外泌体生物学和医学研究和开发的EV样品的定量和定性信息提供有用且易于使用的解决方案。
A microcapillary chip-based particle electrophoresis system developed for characterizing extracellular vesicles (EVs) is described. So far, it is technologically difficult to analyze or identify a heterogeneous population of particles ranging from several tens to one hundred nanometers, and hence, there is a growing demand for a new analytical method of nanoparticles among researchers working on extracellular vesicles. The analytical platformpresented in this chapter allows detection of individual nanoparticles or nanovesicles of less than 50 nm in diameter and enables the characterization of nanoparticles based on multiple indexes such as concentration, diameter, zeta potential, and surface antigenicity. This platform will provide a useful and easy-to-use solution for obtaining both quantitative and qualitative information on EV samples used in research and development of exosome biology and medicine.