Contribution of whole-cell optimization via cell body rolling to polarization of T cells
Contribution of whole-cell optimization via cell body rolling to polarization of T cells
复制标题
DOI：
10.1088/1478-3975/3/3/006
复制
发表时间：
2006-09-01
期刊：
影响因子：
2
通讯作者：
Maly, Ivan V.
中科院分区：
文献类型：
--
作者：
Arkhipov, Sergey N.;Maly, Ivan V.
免疫反应期间 T 细胞定向分泌细胞毒素或细胞因子取决于 T 细胞中的中心体迁移到与靶细胞的界面。中心体易位的机制一直难以捉摸。所提出的计算分析表明，如果 T 细胞同时尝试 (a) 最小化其表面积，(b) 最大化界面面积，(c) 维持细胞体积和 (d) 拉直微管，则中心体应位于界面处。实时三维显微镜和测量表明，中心体的最佳位置很大程度上（约 40%）是通过整个 T 细胞体在目标表面上的滚动来实现的；这种运动似乎带动了中心体。理论和实验结果引起了人们对全细胞结构和全细胞运动在 T 细胞极化中先前未被认识的作用的关注。
Directed secretion of cytotoxins or cytokines by T cells during immune response depends on migration of the centrosome in the T cell to the interface with the target cell. The mechanism of the centrosome translocation has been elusive. The presented computational analysis demonstrates that the centrosome should be positioned at the interface if the T cell attempts simultaneously (a) to minimize its surface area, (b) to maximize the interface area, (c) to maintain the cell volume and (d) to straighten the microtubules. Live three-dimensional microscopy and measurements show that the optimal position of the centrosome is achieved in large part (by about 40%) via rolling of the entire T cell body on the target surface; this movement appears to entrain the centrosome. The theoretical and experimental results draw attention to the previously unrecognized role of the whole-cell structure and whole-cell movements in the T cell polarization.