Simultaneous determination of fangchinoline and tetrandrine in Stephania tetrandra S. Moore by using 1-alkyl-3-methylimidazolium-based ionic liquids as the RP-HPLC mobile phase additives
Simultaneous determination of fangchinoline and tetrandrine in Stephania tetrandra S. Moore by using 1-alkyl-3-methylimidazolium-based ionic liquids as the RP-HPLC mobile phase additives
复制标题
以1-烷基-3-甲基咪唑基离子液体为RP-HPLC流动相添加剂同时测定防己碱和粉防己碱的含量
DOI：
10.1016/j.aca.2013.01.001
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发表时间：
2013-03-12
影响因子：
6.2
通讯作者：
Zhang, Yongqing
中科院分区：
文献类型：
--
作者：
Tang, Yan;Sun, Ailing;Zhang, Yongqing
以四氟硼酸1-己基-3-甲基咪唑鎓盐为流动相添加剂，建立了反相高效液相色谱（RP-HPLC）同时测定防己碱（FAN）和粉防己碱（TET）含量的方法。使用四种 1-烷基-3-甲基咪唑鎓基离子液体 (IL) 作为流动相添加剂，通过 RP-HPLC 分离 FAN 和 TET。详细研究了烷基长度对咪唑鎓环及其抗衡离子、IL 浓度和流动相 pH 值对 FAN 和 TET 色谱行为的影响。还研究了所提出方法的线性、灵敏度、准确性和重复性。探讨并讨论了以离子液体作为流动相添加剂分离的可能机制。 (C) 2013 Elsevier B.V. 保留所有权利。
A reversed phase high performance liquid chromatography (RP-HPLC) method for simultaneous determination of fangchinoline (FAN) and tetrandrine (TET) in Stephania tetrandra S. Moore was established by using 1-hexyl-3-methylimidazolium tetrafluoroborate as the mobile phase additives in this paper. Four types of 1-alkyl-3-methylimidazolium-based ionic liquids (ILs) were used as additives of the mobile phase to separate FAN and TET by RP-HPLC. The effects of the length of the alkyl group on the imidazolium ring and its counterion, the concentrations of IL and the pH of the mobile phase, which influenced the chromatographic behaviors of FAN and TET, were investigated in detail. The linearity, sensitivity, accuracy and repeatability of the proposed method were also investigated. The probable mechanism of the separation with ILs as the mobile phase additives was explored and discussed. (C) 2013 Elsevier B.V. All rights reserved.