webKnossos: efficient online 3D data annotation for connectomics

webKnossos: efficient online 3D data annotation for connectomics
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DOI:
10.1038/nmeth.4331
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发表时间:
2017-07-01
期刊:
影响因子:
48
通讯作者:
Helmstaedter, Moritz
Helmstaedter, Moritz
中科院分区:
生物学1区
文献类型:
--
作者:
Boergens, Kevin M.;Berning, Manuel;Helmstaedter, Moritz

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我们报告webKnossos,在浏览器中的注释工具的三维电子显微镜数据。webKnossos提供了飞行模式,这是一种单视图自我中心重建方法,使训练有素的注释者群体能够以1.5 +/- 0.6 mm/h的速度重建轴突,以2.1 +/- 0.9 mm/h的速度重建哺乳动物皮层的3D电子显微镜数据中的树突。与目前最先进的工具相比,webKnossos将连接组学的神经突重建加速了4到13倍,从而扩展了未来可以实际映射的连接组的范围。
We report webKnossos, an in-browser annotation tool for 3D electron microscopic data. webKnossos provides flight mode, a single-view egocentric reconstruction method enabling trained annotator crowds to reconstruct at a speed of 1.5 +/- 0.6 mm/h for axons and 2.1 +/- 0.9 mm/h for dendrites in 3D electron microscopic data from mammalian cortex. webKnossos accelerates neurite reconstruction for connectomics by 4- to 13-fold compared with current state-of-the-art tools, thus extending the range of connectomes that can realistically be mapped in the future.