The response of healing corneal epithelium to grooved polymer surfaces
The response of healing corneal epithelium to grooved polymer surfaces
复制标题
DOI：
10.1016/j.biomaterials.2004.05.023
复制
发表时间：
2005-05-01
期刊：
影响因子：
14
通讯作者：
Walboomers, XF
中科院分区：
文献类型：
--
作者：
Evans, MDM;McFarland, GA;Walboomers, XF
角膜上皮伤口通过具有收缩伤口前沿的组织向内生长而快速愈合。可以使用这种伤口愈合过程将用于矫正屈光不正的合成聚合物透镜（可植入接触透镜）结合到角膜中。聚合物表面上的形貌线索可促进上皮组织迁移到前器械表面上。这里，将具有平行凹槽的限定表面几何形状（凹槽和脊宽度为1、2、5和10 μ m;凹槽深度为1和5 μ m）的硅树脂盘植入角膜中并保持在器官培养物中。监测测试表面上上皮组织迁移的性质和速率8天，并使用显微镜和组织学进行评价。无论螺距如何，深槽几何形状引导组织沿槽沿着侧向迁移，但这阻止了伤口前部的收缩并延缓了迁移速率。在任何浅槽几何结构上均未发生引导，但这些允许向内径向迁移，伤口前端收缩，支持迁移率，相当于平面。所测试的几何形状均未促进组织在平坦聚合物表面上方的迁移，并且数据表明平行凹槽可能不是该应用的最佳选择。(C)2004 Elsevier Ltd.保留所有权利。
Corneal epithelial wounds heal rapidly by the inwards growth of tissue with a contracting wound front. A synthetic polymer lens to correct refractive error (an implantable contact lens) could be incorporated into the cornea using this wound healing process. Topographical cues on the polymer surface may facilitate epithelial tissue migration over the anterior device surface. Here, silicone discs with a defined surface geometry of parallel grooves (groove and ridge widths of 1,2,5 and 10 mum; groove depths of 1 and 5 mum) were implanted into corneas and maintained in organ culture. The nature and rate of epithelial tissue migration over the test surfaces was monitored for 8 days and evaluated using microscopy and histology. Irrespective of the pitch, deep groove geometries directed tissue migration laterally along the grooves but this prevented contraction of the wound front and retarded migration rates. No guidance occurred on any of the shallow groove geometries but these allowed inwards radial migration with a contracting wound front and supported migration rates equivalent to a flat surface. None of the geometries tested promoted tissue migration above a flat polymer surface and data suggested that parallel grooves may not be optimal for this application. (C) 2004 Elsevier Ltd. All rights reserved.