Image reconstruction and enhancement by deconvolution in scatter-plate microscopy.
Image reconstruction and enhancement by deconvolution in scatter-plate microscopy.
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DOI：
10.1364/oe.27.023049
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发表时间：
2019-08
期刊：
影响因子：
3.8
通讯作者：
S. Ludwig;B. Le Teurnier;G. Pedrini;Xiang Peng;W. Osten
中科院分区：
文献类型：
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作者：
S. Ludwig;B. Le Teurnier;G. Pedrini;Xiang Peng;W. Osten
我们研究了反褶积的能力，图像增强在散射板显微镜。这种无透镜成像技术通过散射介质的散射光强度与先前记录的散射介质的点扩散函数（PSF）相互关联，使微结构研究成为可能。PSF的自相关函数表现为成像过程的传递函数。反卷积方法利用这种传递函数的知识，通过减少模糊和增强与物镜的对比度来提高图像质量，以达到衍射限制的分辨率。我们通过反滤波和迭代反卷积算法获得了显著的图像增强。
We investigated the capabilities of deconvolution for image enhancement in scatter-plate microscopy. This lensless imaging technique enables the investigation of microstructures through scattering media by cross-correlating the scattered light intensity with a previously recorded point spread function (PSF) of the scattering medium. The autocorrelation function of the PSF appears as the transfer function of the imaging process. Deconvolution methods use the knowledge of this transfer function to enhance the image quality by reducing the blur and strengthening the contrast with the objective to achieve diffraction-limited resolution. We obtained significant image enhancement both with means of inverse filtering and by applying iterative deconvolution algorithms.