Z-RNA - A LEFT-HANDED RNA DOUBLE HELIX
Z-RNA - A LEFT-HANDED RNA DOUBLE HELIX
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DOI：
10.1038/311584a0
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发表时间：
1984-01-01
期刊：
影响因子：
64.8
通讯作者：
VANDESANDE, JH
中科院分区：
文献类型：
--
作者：
HALL, K;CRUZ, P;VANDESANDE, JH
与双链DNA相反，到目前为止，几乎没有证据表明双链RNA可以经历重大的构象转变。用核磁共振、圆二色谱和吸收光谱研究了双链RNA分子Poly(G-C)·Poly(G-C)在不同条件下的构象。我们报告了通过这些不同的光谱技术获得的证据，即Poly(G-C)·Poly(G-C)在高离子强度条件下和35°C以上的温度下经历了从A型到左手Z型的转变。这种构象转变可能与双链RNA发生的生物学情况有关，例如在核糖体和一些病毒中。
In contrast to double-stranded DNA, there has so far been little evidence that double-stranded RNA can undergo major conformational transitions. We have investigated the conformation in different conditions of the double-stranded RNA molecule poly(G-C)·poly(G-C), by NMR, circular dichroism and absorbance spectroscopy. We report here evidence obtained by these different spectroscopic techniques that poly(G-C)·poly(G-C) undergoes a transition from the A-form to a left-handed Z-form in conditions of high ionic strength and at temperatures above 35°C. This conformational transition may be of relevance to the biological situations in which double-stranded RNA occurs, such as in ribosomes and in some viruses.