Salidroside rescued mice from experimental sepsis through anti-inflammatory and anti-apoptosis effects
Salidroside rescued mice from experimental sepsis through anti-inflammatory and anti-apoptosis effects
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红景天苷通过抗炎和抗凋亡作用使小鼠免于实验性脓毒症
DOI：
10.1016/j.jss.2015.01.021
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发表时间：
2015-05-01
影响因子：
2.2
通讯作者：
Deng, Xiaoming
中科院分区：
文献类型：
--
作者：
Liu, Shanshan;Yu, Xiongwei;Deng, Xiaoming
背景：红景天苷（Salidroside，SDS）是红景天的主要有效成分，具有多种药理活性。本研究旨在探讨SDS对实验性脓毒症小鼠的治疗作用及其可能的作用机制。方法：采用盲肠结扎穿孔法（CLP）建立C57 BL/6雄性小鼠脓毒症模型。将动物分为以下三组：假手术组、CLP组和CLP + SDS组。术后1h腹腔注射SDS（50 mg/kg）。观察小鼠术后存活率、血液和腹腔灌洗液中细菌清除率、血液中细胞因子分泌量及肺组织学变化。此外，在脾脏和胸腺中的免疫细胞凋亡进行了检查，分别。结果：SDS给药延长脓毒症小鼠的生存，抑制炎症反应，并提高细菌清除。结论：SDS对CLP诱导的脓毒症具有保护作用，其机制可能与减轻炎症反应、增强细菌清除、保护获得性免疫有关。SDS可能是治疗脓毒症的一种有前途的治疗策略。(C)2015 Elsevier Inc. All rights reserved.
Background: Salidroside (SDS) is the main effective component of Rhodiola rosea L with a variety of pharmacologic properties. The objective of this study was to investigate the efficacy of SDS in the treatment of experimental sepsis in mice and explore the possible underlying action mechanisms.Methods: Sepsis was induced in C57BL/6 male mice via cecal ligation and puncture (CLP). The animals were divided into three groups as follows: sham, CLP, and CLP plus SDS. SDS (50 mg/kg) was injected intraperitoneally 1 h after operation. Postoperative survival of the mice, bacterial clearance in blood and peritoneal lavage fluid, cytokine secretion in blood, and histology of lung were evaluated. In addition, apoptosis of immune cells in the spleen and thymus were examined, respectively.Results: SDS administration prolonged the survival of the septic mice, inhibited the proinflammatory responses, and enhanced bacterial clearance. It also alleviated the pathologic changes in the lung and inhibited the apoptosis of immune cells in the spleen and thymus after CLP challenge.Conclusions: SDS exerts a protective effect in CLP-induced sepsis by attenuating the proinflammatory responses, enhancing bacterial clearance, and preserving adaptive immunity. SDS may be a promising therapeutic strategy for the treatment of sepsis. (C) 2015 Elsevier Inc. All rights reserved.