Simulated microgravity using a rotary cell culture system promotes chondrogenesis of human adipose-derived mesenchymal stem cells via the p38 MAPK pathway.
Simulated microgravity using a rotary cell culture system promotes chondrogenesis of human adipose-derived mesenchymal stem cells via the p38 MAPK pathway.
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DOI：
10.1016/j.bbrc.2011.09.103
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发表时间：
2011-10
影响因子：
3.1
通讯作者：
Bo Yu;Degang Yu;Lei Cao;Xin Zhao;Teng Long;Guangwang Liu;Ting‐ting Tang;Zhen’an Zhu
中科院分区：
文献类型：
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作者：
Bo Yu;Degang Yu;Lei Cao;Xin Zhao;Teng Long;Guangwang Liu;Ting‐ting Tang;Zhen’an Zhu
骨髓间充质干细胞（Mesenchymal stem cells，MSCs）具有多向分化潜能，其软骨形成受机械刺激的影响。本研究的目的是使用旋转细胞培养系统（RCCS）生物反应器，研究微重力对人脂肪来源的间充质干细胞（ADSCs）软骨分化的影响，ADSCs在有或没有软骨生长因子TGF-β1的颗粒中培养。此外，我们评估了p38 MAPK通路在这一过程中的作用。实时荧光定量PCR和组织学结果表明，微重力与TGF-β1对软骨形成具有协同作用。p38 MAPK通路仅被TGF-β1激活，并进一步被微重力刺激。用SB 203580抑制p38活性抑制软骨细胞特异性基因表达和基质产生。这些结果表明，p38 MAPK信号作为一个重要的调解人在微重力诱导的ADSCs的软骨形成。
Mesenchymal stem cells (MSCs) are multi-potent, and the chondrogenesis of MSCs is affected by mechanical stimulation. The aim of this study was to investigate, using a rotary cell culture system (RCCS) bioreactor, the effects of microgravity on the chondrogenic differentiation of human adipose-derived MSCs (ADSCs), which were cultured in pellets with or without the chondrogenic growth factor TGF-β1. In addition, we evaluated the role of the p38 MAPK pathway in this process. The real-time PCR and histological results show that microgravity has a synergistic effect on chondrogenesis with TGF-β1. The p38 MAPK pathway was activated by TGF-β1 alone and was further stimulated by microgravity. Inhibition of p38 activity with SB203580 suppressed chondrocyte-specific gene expression and matrix production. These findings suggest that the p38 MAPK signal acts as an essential mediator in the microgravity-induced chondrogenesis of ADSCs.