Rejection of bone marrow cell allografts by natural killer cell subsets: 5E6+ cell specificity for Hh-1 determinant 2 shared by H-2d and H-2f.
Rejection of bone marrow cell allografts by natural killer cell subsets: 5E6+ cell specificity for Hh-1 determinant 2 shared by H-2d and H-2f.
复制标题
自然杀伤细胞亚群排斥骨髓细胞同种异体移植物：5E6 细胞对 Hh-1 决定簇 2 具有 H-2d 和 H-2f 共有的特异性。
DOI：
10.1002/eji.1830211125
复制
发表时间：
1991
影响因子：
5.4
通讯作者：
Bennett,M
中科院分区：
文献类型：
--
作者：
Sentman,CL;Kumar,V;Bennett,M
由抗5E6单抗定义的5E6抗原在一半的小鼠自然杀伤（NK）细胞上表达，我们之前已经证明（CL Sentman et al.， J. Exp. Med. 1989）。170: 1991)发现5E6+ NK细胞对BALB/c （Hh-1 d）的排斥反应是必需的，而不是C567BL/6 （Hh-1 b）骨髓细胞（BMC）的排斥反应。在这里描述的实验中，我们已经表征了5E6+和5E6−NK细胞亚群对造血组织相容性-1 （Hh-1）抗原的特异性。用抗5e6单抗治疗预期受体小鼠，并用来自不同供体的BMC刺激。此外，将H-2 d/Hh-1 d CB-17 scid 5E6+或5E6 - NK细胞过继转移到辐照过的NK细胞枯竭的宿主中，并用H-2 b/Hh-1 b BMC攻毒。这些数据表明，5E6+ NK细胞只对那些表达与菌株BALB/c (d)、A. Ca (f)和B10的h- 2d和h- 2f单倍型共有的Hh-1决定因子2的BMC有排斥作用。米(f)。5E6+人群对其他Hh-1抗原无反应性。相反，NK细胞裂解h - 2d或h - 2b肿瘤细胞的能力与5E6的表达无关。这些结果表明，5E6分子可能在表达Hh-1决定因素2的BMC的特异性识别和排斥中起重要作用，而可能不参与“肿瘤靶细胞结构”的识别。
The 5E6 antigen, defined by anti-5E6 mAb, is expressed on one-half of murine natural killer (NK) cells, and we have previously demonstrated (CL Sentman et al., J. Exp. Med. 1989. 170: 1991) that 5E6+ NK cells are necessary for the rejection of BALB/c (Hh-1 d) but not C567BL/6 (Hh-1 b) bone marrow cells (BMC). In experiments described here, we have characterized the specificity of 5E6+ and 5E6− NK cell subsets for hemopoietic histocompatibility-1 (Hh-1) antigens. Prospective recipient mice were treated with anti-5E6 mAb and challenged with BMC from a variety of donors. In addition, H-2 d/Hh-1 d CB-17 scid 5E6+ or 5E6− NK cells were adoptively transferred into irradiated, NK cell-depleted hosts and challenged with H-2 b/Hh-1 b BMC. The data indicate that the 5E6+ NK cells are necessary for the rejection of only those BMC that express the Hh-1 determinant 2 shared by H-2 d and H-2 f haplotypes of strains BALB/c (d), A. Ca (f), and B10. M (f). No reactivity to other Hh-1 antigens resides in the 5E6+ population. In contrast, the ability of NK cells to lyse H-2 d or H-2 b tumor cells was independent of 5E6 expression. These results suggest that the 5E6 molecule is likely to be important in the specific recognition and rejection of BMC that express Hh-1 determinant 2, and is probably not involved in recognition of „tumor target cell structures”.