Acclimatisation of Fusarium langsethiae, F. poae and F. sporotrichioides to elevated CO2: Impact on fungal growth and mycotoxin production on oat-based media
Acclimatisation of Fusarium langsethiae, F. poae and F. sporotrichioides to elevated CO2: Impact on fungal growth and mycotoxin production on oat-based media
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Fusarium langsethiae、F. poae 和 F. sporotrichioides 对升高的 CO2 的适应：对燕麦培养基上真菌生长和霉菌毒素产生的影响
DOI：
10.1016/j.ijfoodmicro.2023.110176
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发表时间：
2023
影响因子：
5.4
通讯作者：
Kahla A
中科院分区：
文献类型：
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作者：
Kahla A
燕麦对镰刀菌属（Fusariumspecies），尤其是F. langsethiae，F. PoaeandF. sporotrichioides孢子类真菌污染粮食与霉菌毒素。气候变化预计会影响真菌定植和相关的真菌毒素生产。本研究的目的是研究驯化的影响，以提高CO2对这些真菌的生长和真菌毒素生产能力。F. langsethiae（FL; 7株）、F. poae（FP; 2个菌株）和F.拟孢毛孢（FS;通过在400或1000 ppm CO2下在昼夜温度条件下传代培养10代来适应。在每次传代培养时，评估了适应CO2浓度升高对（a）生长前停滞期（B）燕麦基培养基上生长速率的影响。此外，使用LC-MS/MS qTRAP评估了第1、7和10代后传代培养物的A型二孢霉烯和相关毒性次级代谢产物的产生。结果表明，在继代培养和CO2浓度升高的共同作用下，镰刀菌菌株的生长滞后时间和生长速率均有所增加。与环境条件相比，菌株FL 4中CO2升高（增加7.1倍）和菌株FL 1中CO2降低（降低2.0倍）以及7次传代培养后FS（降低1.3倍）影响T-2 + HT-2产量。继代培养对T-2 + HT-2产量的影响因真菌菌株而异。对于菌株FL 4，与在高CO2条件下传代培养一次相比，在10代后产生的T-2 + HT-2毒素显著减少（减少4.4倍），并且在环境条件下没有观察到变化。FS菌株在高CO2条件下传代培养10代后，与该菌株的初始传代培养相比，T-2 + HT-2毒素产生显著刺激（增加1.1倍）。其他毒性次级代谢产物的产生通常不受CO2升高条件或传代培养10代的影响，但FL 1和FP 1除外。FL 1产生显着更多的新茄醇后，10代，相比后1和7，无论CO2条件。对于FP 1，升高的CO2显着触发白僵菌素生产后，初始的继代培养相比，在相同的继代培养阶段（29倍）的环境条件。FP 1适应CO2浓度升高导致白僵菌素生产减少10代后相比，1（6倍）。与此相反，在环境CO2条件下传代培养10代与1代相比，导致该毒素增加（12倍）。
Oats are highly susceptible to infection byFusariumspecies, especiallyF. langsethiae,F. poaeandF. sporotrichioideswhich contaminate the grain with mycotoxins. Climate change is expected to affect fungal colonisation and associated mycotoxin production. The objective of this study was to examine the effect of acclimatisation to elevated CO2on the growth and mycotoxin production capacity of these fungal species. Strains ofF. langsethiae(FL;seven strains),F. poae(FP;two strains) andF. sporotrichioides(FS;one strain) were acclimatised by sub-culturing for 10 generations at either 400 or 1000 ppm CO2under diurnal temperature conditions. At each sub-culturing, the effect of acclimatisation to elevated CO2on (a) lag phase prior to growth, (b) growth rate on oat-based media was assessed. Additionally, the production of type A trichothecenes and related toxic secondary metabolites of sub-cultures after 1, 7 and 10 generations were assessed using LC-MS/MS qTRAP. The results showed thatFusariumstrains had an increased lag time and growth rate in response to the combined effect of sub-culturing and elevated CO2levels. T-2 + HT-2 production was affected by elevated CO2in strain FL4 (7.1-fold increase) and a decrease in strain FL1 (2.0-fold decrease) at the first sub-culturing and FS (1.3-fold decrease) after 7 sub-cultures compared to ambient conditions. The effect of sub-culturing on T-2 + HT-2 production varied depending on the fungal strain. For strain FL4, significantly less T-2 + HT-2 toxins were produced after 10 generations (4.4-fold decrease) as compared to that under elevated CO2conditions after one sub-culture, and no change was observed under ambient conditions. The FS strain showed significant stimulation of T-2 + HT-2 toxin production after 10 sub-cultured generations (1.1-fold increase) compared to the initial sub-culture of this strain under elevated CO2conditions. The production of other toxic secondary metabolites was generally not impacted by elevated CO2conditions or by sub-culture for 10 generations, with the exceptions of FL1 and FP1. FL1 produced significantly more neosolaniol after 10 generations, when compared to those after 1 and 7, regardless of the CO2conditions. For FP1, elevated CO2significantly triggered beauvericin production after an initial sub-culture when compared to ambient conditions at the same sub-culture stage (29-fold). FP1 acclimatisation to elevated CO2led to a decrease of beauvericin production after 10 generations when compared to 1 (6-fold). In contrast, sub-culturing for 10 generations compared to 1 under ambient CO2conditions resulted in an increase in this toxin (12-fold).