Identification of a novel crustacean vascular endothelial growth factor b-like in the mud crab Scylla paramamosain, and examination of its role in lipid accumulation
Identification of a novel crustacean vascular endothelial growth factor b-like in the mud crab Scylla paramamosain, and examination of its role in lipid accumulation
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DOI：
10.1016/j.aquaculture.2023.739793
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发表时间：
2023-10
期刊：
影响因子：
4.5
通讯作者：
Wenjie Zhong;Chuangsi Chen;Senyue Tan;Xianda He;Shuqi Wang;D. Tocher;Fan Lin;Zhijie Sun;
中科院分区：
文献类型：
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作者：
Wenjie Zhong;Chuangsi Chen;Senyue Tan;Xianda He;Shuqi Wang;D. Tocher;Fan Lin;Zhijie Sun;
血管内皮生长因子B（VEGFB）与VEGF家族的其他成员如VEGFA不同，在哺乳动物中仅显示出有限的血管生成潜力，但参与脂质代谢，特别是将存在于循环中的膳食脂质通过血管内皮转运至外周组织。然而，Vegfb在无脊椎动物如具有开放循环系统的十足类甲壳动物中的作用仍然知之甚少。本文首次从十足类甲壳动物拟穴青蟹中克隆了avegfb-like基因的cDNA。该基因编码386个氨基酸，具有Vegf家族的所有典型特征，与其他甲壳动物的Vegf同源性较高。vegfb样基因在鳃、心脏、肌肉等代谢活跃组织中的相对表达量较高，在雌蟹卵巢中的相对表达量随卵巢成熟而增加。体内敲除vegfb样基因可降低血淋巴和肝胰腺中甘油三酯含量，以及肝胰腺中总脂质含量。此外，vegfb-like基因的敲除还增加了长链酰基辅酶A合成酶3/4（acsl 3/4）、脂肪酸转运蛋白1（fatp 1）、脂肪酸敏感脂肪酶（hsl）等脂肪酸转运、分配和水解相关基因的组织表达水平，降低了乙酰辅酶A羧化酶（acc）等脂肪酸合成相关基因的表达水平。结果表明，Vegfb可能在调节甲壳动物脂肪酸的吸收、利用和分配中发挥重要作用。
Vascular endothelial growth factor B (VEGFB), unlike other members of the VEGF family such as VEGFA, shows only limited potential in angiogenesis in mammals, but is involved in lipid metabolism, especially in transporting dietary lipids present in circulation through the vascular endothelium to peripheral tissues. However, the role of Vegfb in invertebrates such as decapod crustaceans with an open circulatory system is still poorly understood. Here, cDNA of avegfb-like gene was cloned and characterized for the first time from a decapod crustacean, mud crabScylla paramamosain. The mud crabvegfb-like gene encoded a polypeptide of 386 amino acids that possessed all the typical features of the Vegf family, and shared high homology with other crustacean Vegf orthologs. Relative expression levels of thevegfb-like gene were higher in metabolically active tissues such as gill, heart and muscle, and increased in ovary of female crabs as ovary matured. Knockdown of thevegfb-like genein vivoreduced the contents of triglyceride in hemolymph and hepatopancreas, as well as total lipid in hepatopancreas. In addition, knockdown of thevegfb-like gene increased tissue expression levels of genes related to fatty acid transportation, allocation and hydrolysis, such as long-chain acyl-CoA synthetase 3/4 (acsl3/4), fatty acid transport protein 1 (fatp1), and hormone-sensitive lipase (hsl), and decreased the expression levels of the genes related to fatty acid synthesis like acetyl-CoA carboxylase (acc). These results in mud crab suggest that Vegfb might play important roles in regulating fatty acid absorption, utilization and allocation in crustaceans.