SPP1 Is Expressed in Corticospinal Neurons of the Macaque Sensorimotor Cortex
SPP1 Is Expressed in Corticospinal Neurons of the Macaque Sensorimotor Cortex
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DOI：
10.1002/cne.22356
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发表时间：
2010-07-01
影响因子：
2.5
通讯作者：
Kojima, Toshio
中科院分区：
文献类型：
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作者：
Higo, Noriyuki;Sato, Akira;Kojima, Toshio
我们最近在恒河猴、普通狨猴和大鼠大脑中检查了 SPP1 的细胞分布，该基因在猕猴初级运动皮层中的表达高于在前运动皮层或前额叶皮层中的表达。原位杂交组织化学显示，SPP1 mRNA 在恒河猴感觉运动皮层 V 层锥体神经元中特异性表达。根据皮质脊髓神经元的分布，这些 SPP1 mRNA 阳性神经元在初级运动区最丰富，其次是 Brodmann 5 区和辅助运动区。此外，将逆行神经解剖示踪剂注射到脊髓的外侧皮质脊髓束（CST）引起SPP1阳性神经元的标记，表明皮质脊髓神经元中SPP1的表达。 SPP1也在恒河猴的丘脑、脑干和脊髓腹角中表达。尽管在狨猴和大鼠的脑干和脊髓中也检测到了SPP1，但在它们的大脑皮层中并未检测到SPP1。恒河猴感觉运动皮层的皮质脊髓神经元中的选择性表达表明，SPP1 在某些灵长类动物高度发达的皮质脊髓系统的功能或结构特化中发挥着关键作用。 J.Comp。内罗尔。 518:2633-2644, 2010。(C)2010 Wiley-Liss, Inc.
The cellular distribution of SPP1, which we recently identified as a gene with greater expression in the macaque primary motor cortex than in the premotor or prefrontal cortices, was examined in rhesus macaque, common marmoset, and rat brains. In situ hybridization histochemistry revealed that SPP1 mRNA was expressed specifically in pyramidal neurons in layer V of the sensorimotor cortex of the rhesus macaque. These SPP1 mRNA-positive neurons were most abundant in the primary motor area, followed by Brodmann area 5 and the supplementary motor area, in accordance with the distribution of corticospinal neurons. In addition, injection of a retrograde neuroanatomical tracer into the lateral corticospinal tract (CST) of the spinal cord caused labeling of SPP1 positive neurons, indicating the expression of SPP1 in corticospinal neurons. SPP1 was also expressed in the thalamus, brainstem, and spinal ventral horn of the rhesus macaque. Although SPP1 was also detected in the brainstem and spinal cord of the marmoset and the rat, it was not detected in their cerebral cortices. Selective expression in the corticospinal neurons of the sensorimotor cortex of the rhesus macaque suggests that SPP1 plays a critical role in the functional or structural specialization of highly developed corticospinal systems in certain primate species. J. Comp. Neurol. 518:2633-2644, 2010. (C)2010 Wiley-Liss, Inc.