Metal ion-directed cooperative DNA binding of small molecules
Metal ion-directed cooperative DNA binding of small molecules
复制标题
DOI：
10.1016/j.jinorgbio.2006.06.008
复制
发表时间：
2006-11-01
影响因子：
3.9
通讯作者：
Jyo, Akinori
中科院分区：
文献类型：
--
作者：
Ihara, Toshihiro;Ikegami, Takashi;Jyo, Akinori
化合物(1)由一个氧基和一个吡啶基组成，是一种金属反应性的DNA结合配体。两个Is与Cu（II）配位形成稳定的二聚体（I-2-Cu），即使在DNA存在的情况下也是如此。在形成二聚体后，I与超声小牛胸腺DNA的结合增强了约10(3)倍；在半摩尔Cu（II）存在和不存在时，其结合常数分别为3.2 × 10(4) M-1和2.4 × 10(7) M-1。结合的巨大加速部分归因于二聚体形成时产生的指示电荷。二聚体之间的高协同性也可能是原因之一；二聚体会沿着双聚体作为模板聚集，形成一维螺旋聚集体。(c) 2006爱思唯尔公司版权所有。
Compound (1), which consists of an oxine and a pyridinium group, was synthesized as a metal-responsive DNA binding ligand. Two Is coordinate to a Cu(II) to form a stable dimer (I-2-Cu), even in the presence of DNA. The binding of I with sonicated calf thymus DNA was enhanced by ca. 10(3) times after forming the dimer; the binding constants were estimated to be 3.2 x 10(4) M-1 and 2.4 x 10(7) M-1 in the absence and the presence, respectively, of a half mole of Cu(II). The enormous acceleration of the binding is partly attributed to the generation of a dicationic charge by the formation of the dimer. High cooperativity between dimers could be also responsible; dimers would gather along the duplex as a template to form I D spiral aggregates. (c) 2006 Elsevier Inc. All rights reserved.