Minor proteins and enzymes of the Drosophila eggshell matrix
Minor proteins and enzymes of the Drosophila eggshell matrix
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DOI：
10.1016/j.ydbio.2006.01.028
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发表时间：
2006-05-01
影响因子：
2.7
通讯作者：
LeMosy, EK
中科院分区：
文献类型：
--
作者：
Fakhouri, M;Elalayli, M;LeMosy, EK
果蝇蛋壳为细胞外基质组装提供了一个体内模型系统，其中程序化基因表达、细胞迁移、细胞外蛋白质运输、蛋白水解加工和交联都是生成多层和区域复杂结构所必需的。虽然蛋壳的丰富结构成分是已知的并且正在被表征。对于可能在蛋壳组装中发挥关键作用的不丰富的结构、调节和酶成分知之甚少。我们使用基于灵敏质谱的分馏蛋壳基质分析来验证六种先前预测的蛋壳蛋白并鉴定出十一种新成分，并表征了许多其 mRNA 的表达模式。其中有几种假定的结构或调节（非酶）蛋白，大多数质量比主要蛋壳蛋白大，并且通常在覆盖蛋壳特定结构特征的卵泡细胞中优先表达。特别值得注意的是假定的酶，其中一些可能参与基质交联（之前涉及蛋壳完整性的两个黄色家族成员、一种血红素过氧化物酶和一种小分子氧化还原酶），另一些可能参与基质蛋白水解或粘附（与组织蛋白酶 B 和 D 相关的蛋白质）。这项工作为未来蛋壳组装的分子研究提供了框架。 (c) 2006 Elsevier Inc. 保留所有权利。
The Drosophila eggshell provides an in vivo model system for extracellular matrix assembly, in which programmed gene expression, cell migrations, extracellular protein trafficking, proteolytic processing, and cross-linking are all required to generate a multi-layered and regionally complex architecture. While abundant structural components of the eggshell are known and are being characterized. less is known about non-abundant structural, regulatory, and enzymatic components that are likely to play critical roles in eggshell assembly. We have used sensitive mass spectrometry-based analyses of fractionated eggshell matrices to validate six previously predicted eggshell proteins and to identify eleven novel components, and have characterized the expression patterns of many of their mRNAs. Among these are several putative structural or regulatory (non-enzymatic) proteins, most larger in mass than the major eggshell proteins and often showing preferential expression in follicle cells overlying specific structural features of the eggshell. Of particular note are the putative enzymes, some likely to be involved in matrix cross-linking (two yellow family members previously implicated in eggshell integrity, a heme peroxidase, and a small-molecule oxidoreductase) and others possibly involved in matrix proteolysis or adhesion (proteins related to cathepsins B and D). This work provides a framework for future molecular studies of eggshell assembly. (c) 2006 Elsevier Inc. All rights reserved.