Engineering high-level aluminum tolerance in barley with the ALMT1 gene
Engineering high-level aluminum tolerance in barley with the ALMT1 gene
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DOI：
10.1073/pnas.0406258101
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发表时间：
2004-10-19
影响因子：
11.1
通讯作者：
Matsumoto, H
中科院分区：
文献类型：
--
作者：
Delhaize, E;Ryan, PR;Matsumoto, H
酸度严重限制了世界上许多农业土壤上的植物产量。有毒的铝(Al)阳离子被酸度溶解，迅速抑制根的生长，并限制随后对水分和养分的吸收。最近的工作表明，小麦(Triticum Aestivum)的ALMT1基因编码一种苹果酸转运蛋白，与苹果酸外排和耐铝性有关。我们获得了表达ALMT1的转基因大麦植株，并评估了它们分泌苹果酸和抵御铝胁迫的能力。ALMT1在大麦中的表达赋予了铝激活的苹果酸外流，其性质类似于耐铝小麦的特性。转基因大麦在水培和酸性土壤上均表现出较高的耐铝性。这些发现为ALMT1是一个主要的耐铝基因提供了进一步的证据，并证明了它通过转基因方法在一个重要的作物物种中赋予对酸性土壤的有效耐性的能力。
Acidity is a serious limitation to plant production on many of the world's agricultural soils. Toxic aluminium (Al) cations solubilized by the acidity rapidly inhibit root growth and limit subsequent uptake of water and nutrients. Recent work has shown that the ALMT1 gene of wheat (Triticum aestivum) encodes a malate transporter that is associated with malate efflux and Al tolerance. We generated transgenic barley (Hordeum vulgare) plants expressing ALMT1 and assessed their ability to exude malate and withstand Al stress. ALMT1 expression in barley conferred an Al-activated efflux of malate with properties similar to those of Al-tolerant wheat. The transgenic barley showed a high level of Al tolerance when grown in both hydroponic culture and on acid soils. These findings provide additional evidence that ALMT1 is a major Al-tolerance gene and demonstrate its ability to confer effective tolerance to acid soils through a transgenic approach in an important crop species.