Leaf stage-associated resistance is correlated with phytohormones in a pathosystem-dependent manner
Leaf stage-associated resistance is correlated with phytohormones in a pathosystem-dependent manner
复制标题
叶期相关抗性以病理系统依赖性方式与植物激素相关
DOI：
10.1111/jipb.12661
复制
发表时间：
2018
影响因子：
11.4
通讯作者：
Cai XZ
中科院分区：
文献类型：
--
作者：
Xu YP;Lv LH;Xu YJ;Yang J;Cao JY;Cai XZ
据报道，在几个病理系统中，抗病性可以在不同阶段的叶片中变化。然而，这种与叶期相关的抗性有多普遍，以及其背后的分子机制在很大程度上仍然未知。在这里，我们研究了叶期对基础抗性，效应触发免疫（ETI）和非宿主抗性的影响，使用八个病理系统，涉及宿主拟南芥，烟草，和N。本氏菌和病原菌核盘菌（Sclerotinia sclerotiorum）、烟粉虱假单胞菌（Pseudomonasingaepv.tabaci）、番茄假单胞菌（P.lingaepv.tomatoDC3000）和水稻黄单胞菌（Xanthomonasoryzaepv. candidae（Xoo））。我们证明了叶期相关抗性在植物中普遍存在，但在不同病理系统的不同阶段具有不同的强度。微阵列表达谱分析表明，数百个基因参与防御反应，植物激素的生物合成和信号，钙信号，在不同时期的叶片之间的差异表达。其中，假单胞菌ssi 1、ssi 2 - 3、ein 2、jar 1 - 1、aba 1和aao 3与缺叶期相关。在与叶期相关的RPS 2/AvrRpt 2+赋予的ETI中，只有突变体aba 1和aba 2 - 3影响与叶期相关的非寄主对Xoo的抗性。我们的研究结果表明，植物激素水杨酸，乙烯，茉莉酸和脱落酸可能发挥了重要的，但病理系统依赖，在叶阶段相关的阻力的作用。
It has been reported in several pathosystems that disease resistance can vary in leaves at different stages. However, how general this leaf stage‐associated resistance is, and the molecular mechanism(s) underlying it, remain largely unknown. Here, we investigated the effect of leaf stage on basal resistance, effector‐triggered immunity (ETI) and nonhost resistance, using eight pathosystems involving the hostsArabidopsis thaliana,Nicotiana tabacum, andN. benthamianaand the pathogensSclerotinia sclerotiorum,Pseudomonas syringaepv.tabaci,P. syringaepv.tomatoDC3000, andXanthomonas oryzaepv.oryzae(Xoo). We show evidence that leaf stage‐associated resistance exists ubiquitously in plants, but with varying intensity at different stages in diverse pathosystems. Microarray expression profiling assays demonstrated that hundreds of genes involved in defense responses, phytohormone biosynthesis and signaling, and calcium signaling, were differentially expressed between leaves at different stages. TheArabidopsismutantssid1,sid2‐3,ein2,jar1‐1,aba1andaao3lost leaf stage‐associated resistance toS. sclerotiorum, and the mutantsaba1andsid2‐3were affected in leaf stage‐associatedRPS2/AvrRpt2+‐conferred ETI, whereas only the mutantsid2‐3influenced leaf stage‐associated nonhost resistance toXoo. Our results reveal that the phytohormones salicylic acid, ethylene, jasmonic acid and abscisic acid likely play an essential, but pathosystem‐dependent, role in leaf stage‐associated resistance.