Comparative Proteomics Reveal Fundamental Structural and Functional Differences between the Two Progeny Phenotypes of a Baculovirus
Comparative Proteomics Reveal Fundamental Structural and Functional Differences between the Two Progeny Phenotypes of a Baculovirus
复制标题
比较蛋白质组学揭示杆状病毒两个子代表型之间的基本结构和功能差异
DOI：
10.1128/jvi.02329-12
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发表时间：
2013-01-01
影响因子：
5.4
通讯作者：
Hu, Zhihong
中科院分区：
文献类型：
--
作者：
Hou, Dianhai;Zhang, Leike;Hu, Zhihong
摘要鳞翅目昆虫杆状病毒的复制以产生两种后代表型为特征：在中肠细胞中建立感染的闭塞衍生病毒(ODV)和将感染传播到敏感宿主内不同组织的芽生病毒(BV)。为了了解BV和ODV在结构和功能上的差异，我们使用了多种蛋白质组学方法来揭示这两种表型棉铃虫核型多角体病毒的蛋白质组成和翻译后修饰。此外，还利用Western blotting和定量质谱仪对蛋白质在包膜或核衣壳部分的定位进行了鉴定。获得了BV和ODV的比较蛋白质组合，显示了54种蛋白质的分布，其中包括BV和ODV共有的21种蛋白质，12种BV特有的蛋白质，以及21种ODV特有的蛋白质。在11种ODV特异性包膜蛋白中，有8种与口腔感染有关或与口腔感染有关。还鉴定了23个磷酸化和6个N-糖基化的病毒蛋白。虽然这两种表型所共有的蛋白质似乎对核衣壳的组装和运输很重要，但这两种表型之间的结构和功能差异明显地表现在包膜蛋白和翻译后修饰上。这项比较蛋白质组学研究为BV和ODV是如何形成的以及为什么它们的功能不同提供了新的见解。
ABSTRACT The replication of lepidopteran baculoviruses is characterized by the production of two progeny phenotypes: the occlusion-derived virus (ODV), which establishes infection in midgut cells, and the budded virus (BV), which disseminates infection to different tissues within a susceptible host. To understand the structural, and hence functional, differences between BV and ODV, we employed multiple proteomic methods to reveal the protein compositions and posttranslational modifications of the two phenotypes of Helicoverpa armigera nucleopolyhedrovirus. In addition, Western blotting and quantitative mass spectrometry were used to identify the localization of proteins in the envelope or nucleocapsid fractions. Comparative protein portfolios of BV and ODV showing the distribution of 54 proteins, encompassing the 21 proteins shared by BV and ODV, the 12 BV-specific proteins, and the 21 ODV-specific proteins, were obtained. Among the 11 ODV-specific envelope proteins, 8 either are essential for or contribute to oral infection. Twenty-three phosphorylated and 6 N-glycosylated viral proteins were also identified. While the proteins that are shared by the two phenotypes appear to be important for nucleocapsid assembly and trafficking, the structural and functional differences between the two phenotypes are evidently characterized by the envelope proteins and posttranslational modifications. This comparative proteomics study provides new insight into how BV and ODV are formed and why they function differently.