Differential expression of an alpha-galactosyl-containing trisaccharide on high- and low-malignant murine sarcoma cells: identification and regulation.
Differential expression of an alpha-galactosyl-containing trisaccharide on high- and low-malignant murine sarcoma cells: identification and regulation.
复制标题
含α-半乳糖基的三糖在高恶性和低恶性鼠肉瘤细胞上的差异表达：识别和调节。
DOI：
10.1023/a:1013865411941
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发表时间：
2002
影响因子：
4
通讯作者：
Goldstein,IrwinJ
中科院分区：
文献类型：
--
作者：
Varani,James;Petryniak,Jerzy;Takagaki,Masaru;Dame,MichaelK;Petryniak,Bronislawa;Goldstein,IrwinJ
Past studies have shown that carbohydrate residues reactive with theGriffonia simplicifoliaisolectin B4(GS I-B4) are present on the surface of highly-malignant murine sarcoma cells but are lacking or expressed in much lower amounts on the surface of low-malignant cells isolated from the same parent tumors (Am J Pathol 111: 27; J Nat Cancer Inst 71: 1281). In the present study it is shown that an antibody which recognizes the trisaccharide Galα1-3Galβ1-4GlcNAc- is reactive with the highly-malignant cells but is non-reactive with the low-malignant cells. Further studies show that the high-malignant cells not only bind GS I-B4but also bindEvonymus europaealectin (which like GS I-B4recognizes terminal galactose in α1-3 linkage) andErythina crystagallilectin (which recognizes sub-terminal galactose in the β1-4 linkage – e.g., Galβ1-4GlcNAc). In contrast, the low malignant cells bindErythina crystagallilectin as efficiently as the high malignant cells but do not bind (or bind much smaller amounts of) either GS I-B4orEvonymus europaealectin. The present studies also show that there is no significant difference between high- and low-malignant cells in expression of α-galactosidase activity. In contrast, the high-malignant cells express high levels of α-galactosyl transferase activity while this enzyme is virtually undetectable in low-malignant cells. Taken together, these studies indicate that differential expression of a single monosaccharide residue distinguishes high- and low-malignant murine sarcoma cells. These studies also identify a mechanism to account for surface carbohydrate differences between the high- and low-malignant cells.
